BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor

科研成果详情

题名	BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor
作者	Wang, Li-Ping 1; Dong, Jin-Zhong 1; Xiong, Li-Jun 2; Shi, Ke-Qing1; Zou, Zhuo-Lin 1; Zhang, Sai-Nan 1; Cao, Su-Ting1; Lin, Zhuo1; Chen, Yong-Ping1
发表日期	2014-12-31
发表期刊	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Carbon tetrachloride liver fibrosis bone morphogenetic protein-7 epidermal growth factor receptor hepatic stellate cell liver fibrosis.
其他关键词	BONE-FORMATION ; FACTOR-BETA ; DEFECTS ; TRANSITION ; MECHANISMS ; MICE
摘要	The aim of this study was to elucidate the effect of bone morphogenetic protein-7 (BMP-7) on liver fibrosis induced by carbon tetrachloride (CCl4) in vivo and on the hepatic stellate cells (HSC) activation in vitro. In vivo, thirty male ICR mice were randomly allocated to three groups, the control group (n = 6), the CCl4 group (n = 18) and the BMP-7+CCl4 group (n = 6). The model of liver fibrosis was induced by intraperitoneal injection with CCl4 three times per week lasting for 12 weeks in CCl4 group and the BMP-7+CCl4 group. After 8 weeks injection with CCl4, mice were intraperitoneal injected with human recombinant BMP-7 in BMP-7+CCl4 group. Meanwhile, mice in the CCl4 group were only intraperitoneal injection with equal amount of saline. The degree of liver fibrosis was assessed by HE and Masson's staining. PCR and western blot were used to detect mRNA and protein levels. In BMP-7+CCl4 group, serum levels of alanine aminotransferase (ALT) and aminotransferase (AST) were decreased and serum albumin (Alb) was increased. Meanwhile, the expressions of transforming growth factor-beta 1 (TGF-beta 1) and alpha-smooth muscle actin (alpha-SMA) were down-regulated by BMP-7 intervention as compared to the CCl4 group (P < 0.05). Furthermore, BMP-7 also suppressed the expression of epidermal growth factor receptor (EGFR) and phosphorylated-epidermal growth factor receptor (pEGFR). HE and Masson stain showed that liver damage was alleviated in BMP-7+CCl4 group. In vitro study, expression of EGFR, TGF-beta 1 and alpha-SMA were down regulated by BMP-7 dose-dependently, indicating it might effect on suppression of HSC activation. Therefore, our data indicate BMP-7 was capable of inhibiting liver fibrosis and suppressing HSCs activation, and these effects might rely on its crosstalk with EGFR and TGF-beta 1. We suggest that BMP-7 may be a potential reagentfor the prevention and treatment of liver fibrosis.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [2013ZX10005002-001]
出版者	E-CENTURY PUBLISHING CORP
出版地	MADISON
ISSN	1936-2625
卷号	7 期号:7 页码:3537-3547
页数	11
WOS类目	Oncology ; Pathology
WOS研究方向	Oncology ; Pathology
WOS记录号	WOS:000341264400004
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	25120732
PMC记录号	PMC4128967
SCOPUSEID	2-s2.0-84906248961
通讯作者地址	[Chen, Yong-Ping]Department of Infection and Liver Diseases, Liver Research Center, The First Affiliated Hospital of Wenzhou Medical University,Nanbaixiang Street,China
Scopus学科分类	Pathology and Forensic Medicine;Histology
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15232
专题	附属第一医院
通讯作者	Chen, Yong-Ping
作者单位	1.Department of Infection Disease, The First Affiliated Hospital of Wenzhou Medical University,China; 2.Department of Pulmonary Medicine, Fuzhou Pulmonary Hospital of Fujian,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wang, Li-Ping,Dong, Jin-Zhong,Xiong, Li-Jun,et al. BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(7):3537-3547.
APA	Wang, Li-Ping., Dong, Jin-Zhong., Xiong, Li-Jun., Shi, Ke-Qing., Zou, Zhuo-Lin., ... & Chen, Yong-Ping. (2014). BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 7(7), 3537-3547.
MLA	Wang, Li-Ping,et al."BMP-7 attenuates liver fibrosis via regulation of epidermal growth factor receptor".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 7.7(2014):3537-3547.