Gradient Elution LC-ESI-MS Determination of Tramadol in Rat Plasma

科研成果详情

题名	Gradient Elution LC-ESI-MS Determination of Tramadol in Rat Plasma
作者	Lin, Chongliang2; Ma, Jianshe3; Lin, Guanyang2; Zhou, Ziye2; Hu, Lufeng2; Wang, Xianqin1
发表日期	2011-10
发表期刊	LATIN AMERICAN JOURNAL OF PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Gradient elution LC-ESI-MS Rat plasma Tramadol
其他关键词	O-DESMETHYLTRAMADOL ; RABBIT PLASMA ; HPLC METHOD ; LIQUID-CHROMATOGRAPHY ; MAIN METABOLITES ; URINE ; HYDROCHLORIDE
摘要	A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of tramadol in rat plasma using one-step protein precipitation was developed. After addition of ketamine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm x 50 mm, 3.5 mu m) column with methanol-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 264.0 for tramadol and m/z 237.8 for the IS. Calibration plots were linear over the range of 5-500 ng/mL for tramadol in rat plasma. Lower limit of quantification (LLOQ) for tramadol was 5 ng/mL. Mean recovery of tramadol from plasma was in the range 92.8 %-97.4 %. RSD of intra-day and inter-day precision were both less than 10 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of tramadol in rat plasma.
出版者	COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES
出版地	LA PLATA
ISSN	0326-2383
卷号	30 期号:8 页码:1500-1505
页数	6
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000298869100007
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84863083064
通讯作者地址	[Wang, Xianqin]Wenzhou Med Coll, Analyt & Testing Ctr, Wenzhou 325035, Peoples R China.
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15181
专题	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）附属第一医院
通讯作者	Wang, Xianqin
作者单位	1.Wenzhou Med Coll, Analyt & Testing Ctr, Wenzhou 325035, Peoples R China; 2.Affiliated Hosp 1, Wenzhou Med Coll, Wenzhou 325035, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Chongliang,Ma, Jianshe,Lin, Guanyang,et al. Gradient Elution LC-ESI-MS Determination of Tramadol in Rat Plasma[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2011,30(8):1500-1505.
APA	Lin, Chongliang, Ma, Jianshe, Lin, Guanyang, Zhou, Ziye, Hu, Lufeng, & Wang, Xianqin. (2011). Gradient Elution LC-ESI-MS Determination of Tramadol in Rat Plasma. LATIN AMERICAN JOURNAL OF PHARMACY, 30(8), 1500-1505.
MLA	Lin, Chongliang,et al."Gradient Elution LC-ESI-MS Determination of Tramadol in Rat Plasma".LATIN AMERICAN JOURNAL OF PHARMACY 30.8(2011):1500-1505.