Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients

doi:10.1167/iovs.61.6.35

科研成果详情

题名	Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients
作者	Kang, Yutong 1; Zhang, Hao 1; Hu, Meina 1; Ma, Yao 1; Chen, Pengfei2; Zhao, Zelin2; Li, Jinyang2; Ye, Yuee2; Zheng, Meiqin1,2; Lou, Yongliang1
发表日期	2020-06
发表期刊	INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	traumatic corneal ulcer ocular surface microbiome shotgun metagenomics
其他关键词	CONJUNCTIVAL MICROBIOME ; BACTERIAL BIOFILMS ; MEIBOMIAN GLAND ; READ ALIGNMENT ; COMMENSAL ; DIVERSITY ; KERATITIS ; SYSTEM ; FLORA ; DNA
摘要	PURPOSE. Corneal ulcers are a common eye inflammatory disease that can cause visual impairment or even blindness if not treated promptly. Ocular trauma is a major risk factor for corneal ulcers, and corneal trauma in agricultural work can rapidly progress to corneal ulcers. This study aims to evaluate the changes in the ocular surface (OS) microbiome of patients with traumatic corneal ulcer (TCU). METHODS. Among 20 healthy control (HC) subjects and 22 patients with TCU, 42 eyes were examined to investigate the OS microbial flora using metagenomic shotgun sequencing. RESULTS. At the taxonomic composition level, our findings showed that dysbiosis (alterations in richness and community structure) occurs in the OS microbiome of patients with TCU. Notably, Pseudomonas was present at a greater than 30% relative abundance in all individuals in the TCU group. At the species level, the abundance of Pseudomonas fluorescens and Pseudomonas aeruginosa was significantly elevated in the TCU group compared to the HC group. At the functional level, we identified significant differences in the HC and TCU groups. We observed that inflammation-related pathways involved in bacterial chemotaxis, flagellar assembly, and biofilm formation were significantly more abundant in the TCU group. Besides, the pathways related to biosynthesis, degradation, and metabolism were also increased significantly in the TCU group. CONCLUSIONS. These findings indicate an altered OS microbiome in the affected eyes of patients with TCU. Further research is needed to determine whether these alterations contribute to the pathogenesis of TCU or impact disease progression.
资助项目	Science and Technology Bureau of Wenzhou [Y20190171]
出版者	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
出版地	ROCKVILLE
ISSN	0146-0404
EISSN	1552-5783
卷号	61 期号:6
DOI	10.1167/iovs.61.6.35
页数	11
WOS类目	Ophthalmology
WOS研究方向	Ophthalmology
WOS记录号	WOS:000544154500036
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	32543662
SCOPUSEID	2-s2.0-85086620622
通讯作者地址	[Zheng, Meiqin]Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Key Laboratory of Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou, Zhejiang,China
Scopus学科分类	Ophthalmology;Sensory Systems;Cellular and Molecular Neuroscience
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15135
专题	眼视光学院（生物医学工程学院）、附属眼视光医院检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Zheng, Meiqin
作者单位	1.Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Key Laboratory of Laboratory Medicine,Ministry of Education China,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 2.School of Ophthalmology and Optometry and Eye Hospital,School of Biomedical Engineering,Wenzhou Medical University,Wenzhou,China
第一作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	模式生物技术与应用重点实验室
推荐引用方式 GB/T 7714	Kang, Yutong,Zhang, Hao,Hu, Meina,et al. Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2020,61(6).
APA	Kang, Yutong., Zhang, Hao., Hu, Meina., Ma, Yao., Chen, Pengfei., ... & Lou, Yongliang. (2020). Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 61(6).
MLA	Kang, Yutong,et al."Alterations in the Ocular Surface Microbiome in Traumatic Corneal Ulcer Patients".INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 61.6(2020).