SDF-1 alpha inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways

doi:10.1007/s11033-010-0071-9

科研成果详情

题名	SDF-1 alpha inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways
作者	Yin, Qi1; Jin, Peifeng1; Liu, Xuebin 2; Wei, Hua ; Lin, Xiaoming1; Chi, Chuang1; Liu, Yu1; Sun, Chengchao1; Wei, Yingjie
发表日期	2011-01
发表期刊	MOLECULAR BIOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	SDF-1 alpha Apoptosis MSCs Hypoxia/SD Signaling pathways
其他关键词	HEMATOPOIETIC PROGENITOR CELLS ; MYOCARDIAL-INFARCTION ; HEART-FAILURE ; CARDIAC REPAIR ; UP-REGULATION ; FACTOR-1-ALPHA ; EXPRESSION ; PROTEIN ; CXCR4 ; REQUIREMENT
摘要	Bone marrow-derived mesenchymal stem cells (BMSCs) have been demonstrated to be a promising cell sources for cardiac regeneration. Poor survival rate of transplanted BMSCs in infarcted myocardium attenuated its clinical application. It's reported that stromal-derived factor-1 (SDF-1) could protect progenitor cells including endothelial progenitor cells and embryonic stem cells from apoptosis. But little is known whether SDF-1 alpha protein has the same protective effects on BMSCs under conditions of hypoxia and serum deprivation (hypoxia/SD). In present study, we verified that SDF-1 alpha (0.50-2.0 mu g/ml) inhibited hypoxia/SD induced apoptosis of BMSCs through mitochondrial pathway. After administration of SDF-1 alpha, the loss of mitochondrial membrane potential and cytochrome c released from mitochondria to cytosol were significantly inhibited, and caspase 3 activity also declined. Furthermore, the effect of SDF-1 alpha on mitochondrial pathway was neutralized by using PI3K inhibitor (Wortmannin) and ERK1/2 inhibitor (U0126). Our observations suggested that SDF-1 alpha inhibits hypoxia/SD induced BMSCs apoptosis through PI3K/Akt and ERK1/2 signaling pathways. These data also imply that the anti-apoptotic effect mediated by SDF-1 alpha may enhance cell survival after cell transplantation.
资助项目	Natural Science Foundation of Wenzhou city, Zhejiang, China [h20090019]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30570722]
出版者	SPRINGER
出版地	DORDRECHT
ISSN	0301-4851
卷号	38 期号:1 页码:9-16
DOI	10.1007/s11033-010-0071-9
页数	8
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000284896200002
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	20383584
SCOPUSEID	2-s2.0-78651107612
通讯作者地址	[Sun, Chengchao]Wenzhou Med Coll, Dept Thorac & Cardiovasc Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15121
专题	附属第一医院_心胸外科
通讯作者	Sun, Chengchao
作者单位	1.Wenzhou Med Coll, Dept Thorac & Cardiovasc Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China; 2.Armed Police Gen Hosp, Res Inst Neural Stem Cell Transplantat, Beijing 100039, Peoples R China
第一作者单位	附属第一医院_心胸外科
通讯作者单位	附属第一医院_心胸外科
第一作者的第一单位	附属第一医院_心胸外科
推荐引用方式 GB/T 7714	Yin, Qi,Jin, Peifeng,Liu, Xuebin,et al. SDF-1 alpha inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways[J]. MOLECULAR BIOLOGY REPORTS,2011,38(1):9-16.
APA	Yin, Qi., Jin, Peifeng., Liu, Xuebin., Wei, Hua., Lin, Xiaoming., ... & Wei, Yingjie. (2011). SDF-1 alpha inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways. MOLECULAR BIOLOGY REPORTS, 38(1), 9-16.
MLA	Yin, Qi,et al."SDF-1 alpha inhibits hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells through PI3K/Akt and ERK1/2 signaling pathways".MOLECULAR BIOLOGY REPORTS 38.1(2011):9-16.