科研成果详情

题名Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)
作者
发表日期2012-02
发表期刊FOLIA PARASITOLOGICA   影响因子和分区
语种英语
原始文献类型Article
关键词Toxoplasma gondii toxoplasmosis loop-mediated isothermal amplification (LAMP) urine B1 mouse
其他关键词POLYMERASE-CHAIN-REACTION ; CONGENITAL TOXOPLASMOSIS ; DNA ; DIAGNOSIS ; PCR ; ENCEPHALITIS ; BLOOD ; BRADYZOITES ; DISEASE ; FLUID
摘要In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The :LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2-6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
资助项目Science and Technology Department of Zhejiang Province of China [2009C33035]
出版者FOLIA PARASITOLOGICA
出版地CESKE BUDEJOVICE
ISSN0015-5683
EISSN1803-6465
卷号59期号:1页码:21-26
DOI10.14411/fp.2012.004
页数6
WOS类目Parasitology
WOS研究方向Parasitology
WOS记录号WOS:000301165000004
收录类别SCIE ; PUBMED ; SCOPUS
URL查看原文
PubMed ID22439424
SCOPUSEID2-s2.0-84863389437
通讯作者地址[Tan, Feng]Wenzhou Med Coll, Dept Parasitol, Wenzhou 325035, Zhejiang, Peoples R China.
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/14952
专题基础医学院(机能实验教学中心)_病原生物学与免疫学系_医学寄生虫学
检验医学院(生命科学学院、生物学实验教学中心)
通讯作者Tan, Feng
作者单位
1.Wenzhou Med Coll, Dept Parasitol, Wenzhou 325035, Zhejiang, Peoples R China;
2.Wenzhou Med Coll, Sch Med Lab Sci, Wenzhou 325035, Zhejiang, Peoples R China;
3.Wenzhou Med Coll, Sch Life Sci, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位温州医科大学;  检验医学院(生命科学学院、生物学实验教学中心)
通讯作者单位温州医科大学
第一作者的第一单位温州医科大学
推荐引用方式
GB/T 7714
Hu, Xin,Pan, Chang-Wang,Li, Ya-Fei,et al. Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)[J]. FOLIA PARASITOLOGICA,2012,59(1):21-26.
APA Hu, Xin, Pan, Chang-Wang, Li, Ya-Fei, Wang, Han, & Tan, Feng. (2012). Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP). FOLIA PARASITOLOGICA, 59(1), 21-26.
MLA Hu, Xin,et al."Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)".FOLIA PARASITOLOGICA 59.1(2012):21-26.

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