Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)

doi:10.14411/fp.2012.004

科研成果详情

题名	Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)
作者	Hu, Xin2,3; Pan, Chang-Wang 1; Li, Ya-Fei 2,3; Wang, Han 1; Tan, Feng1
发表日期	2012-02
发表期刊	FOLIA PARASITOLOGICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Toxoplasma gondii toxoplasmosis loop-mediated isothermal amplification (LAMP) urine B1 mouse
其他关键词	POLYMERASE-CHAIN-REACTION ; CONGENITAL TOXOPLASMOSIS ; DNA ; DIAGNOSIS ; PCR ; ENCEPHALITIS ; BLOOD ; BRADYZOITES ; DISEASE ; FLUID
摘要	In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detection limit of T. gondii LAMP assay was 1 pg of T. gondii DNA, which was evaluated using 10-fold serially diluted DNA of cultured parasites. The :LAMP assay was also highly specific for T. gondii and able to detect T. gondii DNA in urine of mice treated with dexamethasone at 90 day post infection (p.i.), although this assay could not detect the DNA in mice urine 2-6 days p.i. These results demonstrated that LAMP is effective for evaluation of therapy effectiveness for T. gondii infection. The established LAMP assay may represent a useful and practical tool for the routine diagnosis and therapeutic evaluation of human toxoplasmosis.
资助项目	Science and Technology Department of Zhejiang Province of China [2009C33035]
出版者	FOLIA PARASITOLOGICA
出版地	CESKE BUDEJOVICE
ISSN	0015-5683
EISSN	1803-6465
卷号	59 期号:1 页码:21-26
DOI	10.14411/fp.2012.004
页数	6
WOS类目	Parasitology
WOS研究方向	Parasitology
WOS记录号	WOS:000301165000004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22439424
SCOPUSEID	2-s2.0-84863389437
通讯作者地址	[Tan, Feng]Wenzhou Med Coll, Dept Parasitol, Wenzhou 325035, Zhejiang, Peoples R China.
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14952
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Tan, Feng
作者单位	1.Wenzhou Med Coll, Dept Parasitol, Wenzhou 325035, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Sch Med Lab Sci, Wenzhou 325035, Zhejiang, Peoples R China; 3.Wenzhou Med Coll, Sch Life Sci, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	温州医科大学; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Hu, Xin,Pan, Chang-Wang,Li, Ya-Fei,et al. Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)[J]. FOLIA PARASITOLOGICA,2012,59(1):21-26.
APA	Hu, Xin, Pan, Chang-Wang, Li, Ya-Fei, Wang, Han, & Tan, Feng. (2012). Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP). FOLIA PARASITOLOGICA, 59(1), 21-26.
MLA	Hu, Xin,et al."Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification (LAMP)".FOLIA PARASITOLOGICA 59.1(2012):21-26.