Vesicle transport related protein Synaptotagmin-1 mediates paraquat transport to antagonize paraquat toxicity

doi:10.1016/j.tox.2022.153180

科研成果详情

题名	Vesicle transport related protein Synaptotagmin-1 mediates paraquat transport to antagonize paraquat toxicity
作者	Hong, Guang-liang1,2; Tang, Ya-hui1,2; Li, Wen-wen 1,2; Cao, Kai-qiang1,2; Tan, Jia-ping 1,2; Hu, Lu-feng1,2; Chen, Long-wang 1,2; Zhao, Guang-ju1,2; Lu, Zhong-qiu1,2
发表日期	2022-04-30
发表期刊	TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Paraquat Vesicle transport SYT1 Lung injury
其他关键词	CELLS ; CYTOTOXICITY ; MECHANISMS ; EXOCYTOSIS ; APOPTOSIS
摘要	In this study, A549/PQ cells with moderate resistance to paraquat (PQ) were obtained by treating A549 cells with PQ, their growth rate was slowed down, the accumulation concentration of PQ and the levels of growth inhibition, injury and early apoptosis induced by PQ were significantly lower than those of parental A549 cells. Microarray screening and RT-qPCR detection found that Synaptotagmin-1 (SYT1) expression in drug-resistant cells was significantly increased, and PQ further enhanced its expression. After inhibiting SYT1 expression in A549/PQ cells, cell viability, intracellular PQ concentration and the expression of Bcl-2, SNAP25 and RAB26 were significantly reduced, while the mortality, early apoptosis rate and Box expression were significantly increased. In vivo experiments also further showed that PQ promoted the expression of SYT1, SNAP25 and RAB26 in PQ-poisoned mice; when inhibiting SYT1 expression, PQ concentration in lung tissues was significantly increased, and the levels of lung injury and apoptosis were also significantly enhanced, while the expression of SNAP25 and RAB26 was significantly reduced. This indicates that PQ poisoning leads to compensatory upregulation of vesicle transport related proteins such as SYT1 in vivo, thereby promoting PQ transmembrane transport, and then reducing the pulmonary accumulation of PQ and PQ-caused lung injury.
资助项目	National Natural Science Foundation of China [81871550]; Science and Technology Planning Project of Wenzhou City [Y20180104]; Key Specialty of Traditional Chinese Medicine in the 13th Five-Year Plan of Zhejiang Province
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0300-483X
EISSN	1879-3185
卷号	472 页码:153180
DOI	10.1016/j.tox.2022.153180
页数	12
WOS类目	Pharmacology & Pharmacy ; Toxicology
WOS研究方向	Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000790314800002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35430322
SCOPUSEID	2-s2.0-85128289666
通讯作者地址	[Lu, Zhong-qiu]The First Affiliated Hospital of Wenzhou Medical University,Ouhai District, Zhejiang,Wenzhou,325000,China
Scopus学科分类	Toxicology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148470
专题	附属第一医院_急诊科
通讯作者	Lu, Zhong-qiu
作者单位	1.Emergency Department,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Wenzhou Key Laboratory of emergency and disaster medicine,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hong, Guang-liang,Tang, Ya-hui,Li, Wen-wen,et al. Vesicle transport related protein Synaptotagmin-1 mediates paraquat transport to antagonize paraquat toxicity[J]. TOXICOLOGY,2022,472:153180.
APA	Hong, Guang-liang., Tang, Ya-hui., Li, Wen-wen., Cao, Kai-qiang., Tan, Jia-ping., ... & Lu, Zhong-qiu. (2022). Vesicle transport related protein Synaptotagmin-1 mediates paraquat transport to antagonize paraquat toxicity. TOXICOLOGY, 472, 153180.
MLA	Hong, Guang-liang,et al."Vesicle transport related protein Synaptotagmin-1 mediates paraquat transport to antagonize paraquat toxicity".TOXICOLOGY 472(2022):153180.