E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributes to the progression of colon adenocarcinoma

doi:10.1002/jcb.27902

科研成果详情

题名	E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributes to the progression of colon adenocarcinoma
作者	Ye, Yaqun1; Gu, Binbin2; Wang, Yi2; Shen, Sudan2; Huang, Wei 2
发表日期	2019-04
发表期刊	JOURNAL OF CELLULAR BIOCHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	colon adenocarcinoma miRNA-218-5p MNX1-AS1
其他关键词	LONG NONCODING RNA ; CELL-PROLIFERATION ; CANCER ; PROMOTES ; EXPRESSION ; APOPTOSIS ; MIGRATION ; INVASION ; KNOCKDOWN ; PROGNOSIS
摘要	The long noncoding RNA MNX1-AS1 has been reported to facilitate the progression of glioblastoma and ovarian cancer. Nevertheless, the biological roles and underlying mechanisms of MNX1-AS1 in colon adenocarcinoma have not been studied until now. In the current study, MNX1-AS1 was upregulated in colon adenocarcinoma. JASPAR prediction tool showed that E2F1 could bind to the promoter region of MNX1-AS1. The chromatin immunoprecipitation assay and luciferase reporter assay were used to verify the interactions between MNX1-AS1 and E2F1. Then functional assays revealed that downregulation of MNX1-AS1 decreased cell proliferation, migration, and invasion in colon adenocarcinoma, but upregulation of E2F1 reversed the effects. Moreover, subcellular fractionation assay manifested that MNX1-AS1 was enriched in the cytoplasm of colon adenocarcinoma cells, thus we speculated whether MNX1-AS1 could function as a competing endogenous RNA (ceRNA) to play roles in colon adenocarcinoma. Bioinformatics analysis and luciferase reporter assay indicated that MNX1-AS1 could sponge microRNA-218-5p (miR-218-5p). Furthermore, we discovered that SEC61A1 was downstream target of miR-218-5p, and MNX1-AS1 acted as a ceRNA to upregulate the expression of SEC61A1 through sponging miR-218-5p. Finally, rescue assays confirmed that MNX1-AS1 facilitated the progression of colon adenocarcinoma through regulating miR-218-5p/SEC61A1 axis. Taken together, we concluded that E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributed to the progression of colon adenocarcinoma.
资助项目	Wenzhou science and technology project [Y20120030]
出版者	WILEY
出版地	HOBOKEN
ISSN	0730-2312
EISSN	1097-4644
卷号	120 期号:4 页码:6145-6153
DOI	10.1002/jcb.27902
页数	9
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000459010100138
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30362161
SCOPUSEID	2-s2.0-85055515127
通讯作者地址	[Huang, Wei]Nutrition Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Biochemistry;Molecular Biology;Cell Biology
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14846
专题	附属第一医院附属第一医院_营养科
通讯作者	Huang, Wei
作者单位	1.The Operating Room,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Nutrition Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Ye, Yaqun,Gu, Binbin,Wang, Yi,et al. E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributes to the progression of colon adenocarcinoma[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2019,120(4):6145-6153.
APA	Ye, Yaqun, Gu, Binbin, Wang, Yi, Shen, Sudan, & Huang, Wei. (2019). E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributes to the progression of colon adenocarcinoma. JOURNAL OF CELLULAR BIOCHEMISTRY, 120(4), 6145-6153.
MLA	Ye, Yaqun,et al."E2F1-mediated MNX1-AS1-miR-218-5p-SEC61A1 feedback loop contributes to the progression of colon adenocarcinoma".JOURNAL OF CELLULAR BIOCHEMISTRY 120.4(2019):6145-6153.