Diagnostic performance of the metagenomic next-generation sequencing in lung biopsy tissues in patients suspected of having a local pulmonary infection

doi:10.1186/s12890-022-01912-4

科研成果详情

题名	Diagnostic performance of the metagenomic next-generation sequencing in lung biopsy tissues in patients suspected of having a local pulmonary infection
作者	Su, Shan-shan1; Chen, Xue-bing 2; Zhou, Ling-ping1; Lin, Peng-cheng1; Chen, Jun-jie1; Chen, Cheng-shui1; Wu, Qing3; Ye, Jun-ru1; Li, Yu-ping1
发表日期	2022-03-29
发表期刊	BMC PULMONARY MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Local pulmonary infections CT-guided lung biopsy Metagenomic next-generation sequencing Conventional examinations Lung abscess
其他关键词	ASPIRATION ; ABSCESS
摘要	Purpose This study aims to evaluate the diagnostic application and performance of the metagenomic next-generation sequencing (mNGS) in patients suspected of local pulmonary infection by comparing it to the traditional pathogen detection methods in lung tissue specimens obtained by a computerized tomography-guided biopsy (CT-guided biopsy). Methods We retrospectively reviewed patients, admitted to the First Affiliated Hospital of Wenzhou Medical University, China from May 2018 to December 2020, who were suspected of local pulmonary infection. All cases received a CT-guided lung biopsy, tissue samples were sent both for conventional examinations (CE) and mNGS tests. The sensitivity and specificity of the two diagnostic approaches were compared. Results 106 patients enrolled, 76 patients were diagnosed with a pulmonary infection. Among 49 patients with identified pathogens, CE confirmed pathogenic infections in 32 cases. Mycobacterium spp. and fungi accounted for 37.5% (12/32) and 28.1% (9/32), respectively, with bacteria 34.4% (11/32). The mNGS examination detected extra pathogenic microorganisms in 22 patients that were consistent with the patients' clinical and radiographic pictures. The sensitivity of mNGS was 53.9% vs. 42.1% for the CE, while the specificity was 56.7% versus 96.7%. For detection rate, mNGS was significantly superior to CE in bacterial (96.3% vs. 40.7%, p < 0.05), and mixed infections (100% vs. 50%, p < 0.05), but inferior to CE in fungal (60% vs. 90%, p > 0.05) and Mycobacterium spp. infections (66.7% vs. 100%, p > 0.05) with no significant difference. Among 31 cases diagnosed with lung abscess, the diagnostic performance of the detection rate was 67.7% (21/31) in favour of mNGS compared to 29.0% (9/31) for CE (p < 0.05). Most polymicrobial infections were induced by anaerobic species that coexisted with Streptococcus constellatus. And Klebsiella pneumoniae was the most common isolated monomicrobial infection. Conclusions The most commonly detected causative pathogens for local pulmonary infections were bacteria, Mycobacterium spp. and fungi. Compared with the CE, the advantages of mNGS in the pathogens detection lie in the discovery of bacterial and mixed infections, as well as in the detection of lung abscess. Conversely, mNGS is not good enough to be recommendable for the detection of Mycobacterium spp. and fungi.
资助项目	Project of Wenzhou Science and Technology Bureau [Y20210144]
出版者	BMC
出版地	LONDON
ISSN	1471-2466
卷号	22 期号:1 页码:112
DOI	10.1186/s12890-022-01912-4
页数	11
WOS类目	Respiratory System
WOS研究方向	Respiratory System
WOS记录号	WOS:000774939000002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35351079
SCOPUSEID	2-s2.0-85127263287
通讯作者地址	[Li, Yu-ping]The Key Laboratory of Interventional Pulmonology of Zhejiang Province,Department of Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Wenzhou Medical University,South Baixiang, Ouhai District, Zhejiang,Wenzhou,325015,China
Scopus学科分类	Pulmonary and Respiratory Medicine
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148302
专题	第一临床医学院（信息与工程学院）、附属第一医院_内科学_呼吸与危重症医学科附属第一医院_医疗质量管理处
通讯作者	Li, Yu-ping
作者单位	1.The Key Laboratory of Interventional Pulmonology of Zhejiang Province,Department of Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Wenzhou Medical University,South Baixiang, Ouhai District, Zhejiang,Wenzhou,325015,China; 2.Medical Record Statistics Room,Medical Quality Control Department,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325015,China; 3.The Center of Laboratory and Diagnosis,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325015,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Su, Shan-shan,Chen, Xue-bing,Zhou, Ling-ping,et al. Diagnostic performance of the metagenomic next-generation sequencing in lung biopsy tissues in patients suspected of having a local pulmonary infection[J]. BMC PULMONARY MEDICINE,2022,22(1):112.
APA	Su, Shan-shan., Chen, Xue-bing., Zhou, Ling-ping., Lin, Peng-cheng., Chen, Jun-jie., ... & Li, Yu-ping. (2022). Diagnostic performance of the metagenomic next-generation sequencing in lung biopsy tissues in patients suspected of having a local pulmonary infection. BMC PULMONARY MEDICINE, 22(1), 112.
MLA	Su, Shan-shan,et al."Diagnostic performance of the metagenomic next-generation sequencing in lung biopsy tissues in patients suspected of having a local pulmonary infection".BMC PULMONARY MEDICINE 22.1(2022):112.