Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats

doi:10.1016/j.sjbs.2022.02.012

科研成果详情

题名	Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats
作者	Li, Cheng 1; Salmen, Saleh H.2; Alahmadi, Tahani Awad 3; Veeraraghavan, Vishnu Priya 4; Surapaneni, Krishna Mohan 5; Natarajan, Nandakumar 6; Subramanian, Senthilkumar 7
发表日期	2022-05
发表期刊	SAUDI JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	8-Hydroxy-2'-deoxyguanosine Nano-drug delivery Liver cancer Allyl isothiocyanate Diethylnitrosamine
其他关键词	ALLYL ISOTHIOCYANATE ; DNA-DAMAGE ; CELL-PROLIFERATION ; SERUM CEA ; APOPTOSIS ; NANOPARTICLES ; METASTASIS ; HEPATOTOXICITY ; NANOCARRIERS ; PROGRESSION
摘要	Background: Nano-based drug delivery systems have shown several advantages in cancer treatment like specific targeting of cancer cells, good pharmacokinetics, and lesser adverse effects. Liver cancer is a fifth most common cancer and third leading cause of cancer-related mortalities worldwide. Objective: The present study focusses to formulate the selenium (S)/chitosan (C)/polyethylene glycol (Pg)/allyl isothiocyanate (AI) nanocomposites (SCPg-AI-NCs) and assess its therapeutic properties against the diethylnitrosamine (DEN)-induced liver cancer in rats via inhibition of oxidative stress and tumor markers. Methodology: The SCPg-AI-NCs were synthesized by ionic gelation technique and characterized by various characterization techniques. The liver cancer was induced to the rats by injecting a DEN (200 mg/kg) on the 8th day of experiment. Then DEN-induced rats treated with 10 mg/kg of formulated SCPg-AI-NCs an hour before DEN administration for 16 weeks. The 8-hydroxy-2'-deoxyguanosine (8-OHdG) content, albumin, globulin, and total protein were examined by standard methods. The level of glutathione (GSH), vitamin-C & -E, and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were examined using assay kits. The liver marker enzymes i.e., alanine transaminase (ALT), aspartate tansaminase (AST), gamma-glutamyl transaminase (GGT), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activities, alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA), Bax, and Bcl-2 levels, and caspase-3&9 activities was examined using assay kits and the liver histopathology was assessed microscopically by hematoxylin and eosin staining method. The effect of formulated SCPg-AI-NCs on the viability and apoptotic cell death on the HepG2 cells were examined using MTT and dual staining assays, respectively. Results: The results of different characterization studies demonstrated the formation of SCPg-AI-NCs with tetragonal shape, narrowed distribution, and size ranging from 390 to 450 nm. The formulated SCPg-AINCs treated liver cancer rats indicated the reduced levels of 8-OHdG, albumin, globulin, and total protein. The SCPg-AI-NCs treatment appreciably improved the GSH, vitamin-C & -E contents, and SOD, CAT, GPx, and GR activities in the serum of liver cancer rats. The SCPg-AI-NCs treatment remarkably reduced the liver marker enzyme activities in the DEN-induced rats. The SCPg-AI-NCs treatment decreased the AFP and CEA contents and enhanced the Bax and caspase 3&9 activities in the DEN-induced rats. The SCPg-AI-NCs effectively decreased the cell viability and induced apoptosis in the HepG2 cells. Conclusion: The present findings suggested that the formulated SCPg-AI-NCs remarkably inhibited the DEN-induced liver carcinogenesis in rats. These findings provide an evidence that SCPg-AI-NCs can be a promising anticancer nano-drug in the future to treat the liver carcinogenesis. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University.
资助项目	King Saud University, Riyadh, Saudi Arabia [RSP-2021/230]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	1319-562X
EISSN	2213-7106
卷号	29 期号:5 页码:3354-3365
DOI	10.1016/j.sjbs.2022.02.012
页数	12
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000794858400001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	35844425
SCOPUSEID	2-s2.0-85125479105
通讯作者地址	[Subramanian, Senthilkumar]Department of Biochemistry and Physiology,College of Medicine and Health Science,Jigjiga University,Jigjiga,Ethiopia
Scopus学科分类	Agricultural and Biological Sciences (all)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148234
专题	附属第一医院_介入科
通讯作者	Subramanian, Senthilkumar
作者单位	1.Department of Interventional Medicine,The First Affiliated Hospital of Wenzhou Medical University,South Bai Xiang Street, Ouhai District, Zhejiang,Wenzhou,325000,China; 2.Department of Botany and Microbiology,College of Science,King Saud University,PO Box-2455,Riyadh,11451,Saudi Arabia; 3.Department of Pediatrics,College of Medicine and King Khalid University Hospital,King Saud University,Medical City, PO Box-2925,Riyadh,11461,Saudi Arabia; 4.Department of Biochemistry,Saveetha Dental College,Saveetha Institute of Medical and Technical Sciences,Saveetha University,Chennai,600 077,India; 5.Department of Biochemistry,Panimalar Medical College Hospital & Research Institute,Varadharajapuram, Poonamallee,Chennai,600 123,India; 6.Department of Surgery,School of Medicine,UCSF Immunogenetics and Transplantation Laboratory,University of California,San Francisco, 3333, California Street, Suite 150,San Francisco,94118,United States; 7.Department of Biochemistry and Physiology,College of Medicine and Health Science,Jigjiga University,Jigjiga,Ethiopia
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Li, Cheng,Salmen, Saleh H.,Alahmadi, Tahani Awad,et al. Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats[J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES,2022,29(5):3354-3365.
APA	Li, Cheng., Salmen, Saleh H.., Alahmadi, Tahani Awad., Veeraraghavan, Vishnu Priya., Surapaneni, Krishna Mohan., ... & Subramanian, Senthilkumar. (2022). Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats. SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 29(5), 3354-3365.
MLA	Li, Cheng,et al."Anticancer effect of selenium/chitosan/polyethylene glycol/allyl isothiocyanate nanocomposites against diethylnitrosamine-induced liver cancer in rats".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 29.5(2022):3354-3365.