In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing

doi:10.1016/j.toxlet.2022.05.006

科研成果详情

题名	In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing
作者	Deng, Shun 1; Zhang, Enming 2; Wang, Yan 3; Zhao, Yunyang4; Yang, Zezhong 5; Zheng, Bingxin 1; Mu, Xiaoyuan 1; Deng, Xuangen 1; Shen, Hai 1; Rong, Haibo 1; Pei, Desheng 3
发表日期	2022-06-15
发表期刊	TOXICOLOGY LETTERS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Graphene quantum dots Toxicity Zebrafish Transcriptomic responses
其他关键词	ZEBRAFISH LARVAE ; OXIDE ; PERTURBATION ; EXPOSURE ; CHANNELS
摘要	GQDs show great potential in drug carriers, bioimaging, biosensors, theranostics, and are recently reported as promising therapeutic agents to treat amyloid-related diseases such as Parkinson's disease and inflammations such as colitis. However, current toxicity data about GQDs based on in vivo toxicity assessments remain scarce. In the study, we examined the mRNA expression changes of zebrafish embryos exposed to four types of GQDs, including raw graphene quantum dots (R-GQDs), graphene oxide quantum dots (GOQDs), carboxyl GQDs (CGQDs), and aminated GQDs (A-GQDs). Firstly, we treated embryos with the four GQDs at three concentrations (50, 100, and 200 mu g/mL), and found that only A-GQDs caused embryonic developmental arrest at 100 and 200 mu g/mL with significantly decreased survival rates and heartbeat rates, as well as the elevated malformation rates. Next, we analyzed the mRNA sequencing data acquired from zebrafish embryos exposed to the four GQDs for 7 days at 100 mu g/mL, and found that all GQDs can act on potassium (K+) and calcium (Ca2+) channels, and spliceosomes with varying degrees of regulatory effects. Compared to other GQDs, A-GQDs can strongly perturb the anticoagulant protein C (PC) pathway via activating most genes associated with complement and coagulation system, cell adhesion molecules (CAMs), and MAPK. In conclusion, this study provided substantial transcriptomic data underlying the common signaling pathways induced by various types of GQDs and pointed out the specific toxicity of A-GQDs on hemostatic system.
资助项目	Central Government Funds of Guiding Local Scientific and Technological Development for Sichuan Province [2021ZYD0068]; Sichuan Medical Association [S20022]; Fundamental Research Fund for the Central Universities from Ministry of Education, PRC [2015CP001]
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0378-4274
EISSN	1879-3169
卷号	363 页码:55-66
DOI	10.1016/j.toxlet.2022.05.006
页数	12
WOS类目	Toxicology
WOS研究方向	Toxicology
WOS记录号	WOS:000808581100001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35643291
SCOPUSEID	2-s2.0-85131115646
通讯作者地址	[Deng, Shun]Sichuan Provincial Orthopedic Hospital,Chengdu,610041,China
Scopus学科分类	Toxicology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148155
专题	附属第二医院_科研中心
通讯作者	Deng, Shun
作者单位	1.Sichuan Provincial Orthopedic Hospital,Chengdu,610041,China; 2.School of Sports Medicine and Physical Therapy,Beijing Sport University,Beijing,100084,China; 3.Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Science,Chongqing,400714,China; 4.Scientific Research Center,Wenzhou Medical University,Wenzhou,325035,China; 5.Institute of Plant Protection,Tianjin Academy of Agricultural Sciences,Tianjin,300192,China
推荐引用方式 GB/T 7714	Deng, Shun,Zhang, Enming,Wang, Yan,et al. In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing[J]. TOXICOLOGY LETTERS,2022,363:55-66.
APA	Deng, Shun., Zhang, Enming., Wang, Yan., Zhao, Yunyang., Yang, Zezhong., ... & Pei, Desheng. (2022). In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing. TOXICOLOGY LETTERS, 363, 55-66.
MLA	Deng, Shun,et al."In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing".TOXICOLOGY LETTERS 363(2022):55-66.