A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer

doi:10.3389/fonc.2022.873725

科研成果详情

题名	A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer
作者	Hu, Haoran 1; Yang, Meiqin 1; Dong, Wei 1; Yin, Bo 1; Ding, Jianyi 1; Huang, Baoyou2; Zheng, Qingliang 3; Li, Fang 4; Han, Lingfei 1
发表日期	2022-04-29
发表期刊	FRONTIERS IN ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	pyroptosis-related genes panel tumor immune microenvironment cervical cancer prognosis
其他关键词	TUMOR-NECROSIS-FACTOR ; CELL-DEATH ; MACROPHAGES ; CARCINOMA ; ACTIVATION ; EXPRESSION ; CASPASES ; TARGETS ; CD4
摘要	Cervical cancer (CC) is one of the most common malignant tumors of the female reproductive system. And the immune system disorder in patients results in an increasing incidence rate and mortality rate. Pyroptosis is an immune system-related programmed cell death pathway that produces systemic inflammation by releasing pro-inflammatory intracellular components. However, the diagnostic significance of pyroptosis-related genes (PRGs) in CC is still unclear. Therefore, we identified 52 PRGs from the TCGA database and screened three Differentially Expressed Pyroptosis-Related Genes (DEPRGs) in the prognosis of cervical cancer: CHMP4C, GZMB, TNF. The least absolute shrinkage and selection operator (LASSO) regression analysis and multivariate COX regression analysis were then used to construct a gene panel based on the three prognostic DEPRGs. The patients were divided into high-and low-risk groups based on the median risk score of the panel. According to the Kaplan-Meier curve, there was a substantial difference in survival rates between the two groups, with the high-risk group's survival rate being significantly lower than the low-risk group's. The PCA and t-SNE analyses revealed that the panel was able to differentiate patients into high-and low-risk groups. The area under the ROC curve (AUC) shows that the prognostic panel has high sensitivity and specificity. The risk score could then be employed as an independent prognostic factor using univariate and multivariate COX regression analyses paired with clinical data. The analyses of GO and KEGG functional enrichment of differentially expressed genes (DEGs) in the high-and low-risk groups revealed that these genes were primarily engaged in immune response and inflammatory cell chemotaxis. To illustrate immune cell infiltration in CC patients further, we used ssGSEA to compare immune-related cells and immune pathway activation between the high-and low-risk groups. The link between three prognostic DEPRGs and immune-related cells was still being discussed after evaluating immune cell infiltration in the TCGA cohort with CIBERSORT. In addition, the GEPIA database and qRT-PCR analysis were used to verify the expression levels of prognostic DEPRGs. In conclusion, PRGs are critical in tumor immunity and can be utilized to predict the prognosis of CC.
资助项目	National Natural Science Foundation of China [81572546, 81972422, 81771529, 81871167, 82071644]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	2234-943X
卷号	12 页码:873725
DOI	10.3389/fonc.2022.873725
页数	17
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000796384400001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
Pubmed记录号	35574296
Scopus记录号	2-s2.0-85130299942
通讯作者地址	[Han, Lingfei]Department of Gynecology,Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai,China ; [Zheng, Qingliang]Prenatal Diagnosis Center,The Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen,China ; [Li, Fang]Department of Gynecology,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai,China
scopus学科分类	Oncology;Cancer Research
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148048
专题	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学_妇科附属第一医院
通讯作者	Zheng, Qingliang; Li, Fang; Han, Lingfei
作者单位	1.Department of Gynecology,Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai,China; 2.Department of Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Prenatal Diagnosis Center,The Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen,China; 4.Department of Gynecology,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai,China
推荐引用方式 GB/T 7714	Hu, Haoran,Yang, Meiqin,Dong, Wei,et al. A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer[J]. FRONTIERS IN ONCOLOGY,2022,12:873725.
APA	Hu, Haoran., Yang, Meiqin., Dong, Wei., Yin, Bo., Ding, Jianyi., ... & Han, Lingfei. (2022). A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer. FRONTIERS IN ONCOLOGY, 12, 873725.
MLA	Hu, Haoran,et al."A Pyroptosis-Related Gene Panel for Predicting the Prognosis and Immune Microenvironment of Cervical Cancer".FRONTIERS IN ONCOLOGY 12(2022):873725.