EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression

doi:10.1186/s13046-022-02368-4

科研成果详情

题名	EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression
作者	Yang, Meiqin 1; Hu, Haoran 1; Wu, Sufang 2; Ding, Jianyi 1; Yin, Bo 1; Huang, Baoyou3; Li, Fang 4; Guo, Xiaoqing 1; Han, Lingfei 1
发表日期	2022-05-05
发表期刊	JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Circ_0087429 Cervical cancer MiR-5003-3p OGN Epithelial to mesenchymal transition
其他关键词	CIRCULAR RNA ; MESENCHYMAL TRANSITION ; BREAST-CANCER ; SPONGE ; SUPPRESS ; ACTS ; ADENOCARCINOMA ; INVASIVENESS ; RESISTANCE ; LANDSCAPE
摘要	Background Circular RNAs (circRNAs) are noncoding RNAs with stable structures with high expression and tissue-specific expression. Studies have shown that circRNA dysregulation is closely related to the progression of tumours. However, the function and regulatory mechanism of most circRNAs in cervical cancer are still unclear. Methods CircRNAs related to cervical cancer were screened through the Gene Expression Omnibus (GEO) database. qRT-PCR was used to verify the expression of circ_0087429 in cervical cancer tissues and cells. Then, in vivo and in vitro experiments were conducted to evaluate the role of circ_0087429 in the progression of cervical cancer. The role of the circ_0087429/miR-5003-3p/osteoglycin (OGN) axis in the epithelial to mesenchymal transition (EMT) was confirmed by rescue experiments, fluorescence in situ hybridization, luciferase reporter assays, immunofluorescence staining and western blotting. The inhibitory effect of Eukaryotic initiation factor 4A-III (EIF4A3) on the biogenesis of circ_0087429 was verified by RNA immunoprecipitation (RIP) assays and qRT-PCR. Results circ_0087429 is significantly downregulated in cervical cancer tissues and cells and negatively correlated with International Federation of Gynecology and Obstetrics (FIGO) staging and lymphatic metastasis in cervical cancer patients. circ_0087429 can significantly inhibit the proliferation, migration, invasion and angiogenesis of cervical cancer in vitro and tumour growth and metastasis in vivo. OGN is significantly downregulated in cervical cancer tissues and cells. circ_0087429 can upregulate the expression of OGN by competitively binding with miR-5003-3p, thereby reversing EMT and inhibiting the progression of cervical cancer. EIF4A3 can inhibit circ_0087429 expression by binding to its flanking regions. Conclusions As a tumour suppressor, circ_0087429 regulated by EIF4A3 can reverse EMT and inhibit the progression of cervical cancer through the miR-5003-3p/OGN axis. It is expected to become a potential target for the treatment of cervical cancer.
资助项目	National Science Foundation of China [81572546, 81972422, 81771529]
出版者	BMC
出版地	LONDON
ISSN	1756-9966
EISSN	1756-9966
卷号	41 期号:1 页码:165
DOI	10.1186/s13046-022-02368-4
页数	20
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000791363900002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
Pubmed记录号	35513835
Scopus记录号	2-s2.0-85129376649
通讯作者地址	[Guo, Xiaoqing]Department of Gynecology,Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai,200092,China ; [Han, Lingfei]Department of Gynecology,Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai,200092,China ; [Li, Fang]Department of Gynecology,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai,200120,China
scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/147997
专题	第一临床医学院（信息与工程学院）、附属第一医院_妇产科学_妇科附属第一医院
通讯作者	Li, Fang; Guo, Xiaoqing; Han, Lingfei
作者单位	1.Department of Gynecology,Shanghai First Maternity and Infant Hospital,School of Medicine,Tongji University,Shanghai,200092,China; 2.Department of Obstetrics and Gynecology,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,201620,China; 3.Department of Gynecology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang,325027,China; 4.Department of Gynecology,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai,200120,China
推荐引用方式 GB/T 7714	Yang, Meiqin,Hu, Haoran,Wu, Sufang,et al. EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression[J]. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,2022,41(1):165.
APA	Yang, Meiqin., Hu, Haoran., Wu, Sufang., Ding, Jianyi., Yin, Bo., ... & Han, Lingfei. (2022). EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 41(1), 165.
MLA	Yang, Meiqin,et al."EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 41.1(2022):165.