FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway

doi:10.1111/cpr.13221

科研成果详情

题名	FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway
作者	Hu, Zhicheng1,2; Chen, Peng 1; Wang, Linlin3; Zhu, Yu 4; Chen, Gen2,5; Chen, Yunjie 6; Hu, Zhenyu2; Mei, Lin 2; You, Weijing 7; Cong, Weitao2; Jin, Litai2; Wang, Xu 2; Wang, Yang4; Guan, Xueqiang1
发表日期	2022-05
发表期刊	CELL PROLIFERATION 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	CARDIOMYOCYTE PROLIFERATION ; SKELETAL-MUSCLE ; HEART ; FIBROBLASTS ; EXPRESSION ; GROWTH ; REGENERATION
摘要	Objectives Myocardial infarction (MI) commonly occurs in patients with coronary artery disease and have high mortality. Current clinical strategies for MI still limited to reducing the death of myocardial cells but failed to replace these cells. This study aimed to investigate the role of fibroblast growth factor 6 (FGF6) in enhancing the proliferative potential of cardiomyocytes (CMs) after ischemic injury via the Hippo pathway. Materials and Methods Expression of FGF6 protein was analysed in mice with MI induced by ligation of the left anterior descending coronary artery. Activation of the Hippo pathway and the proliferation potential were examined in ischemic CMs, treated with FGF6 protein or transfected with an adeno-virus carrying FGF6 sh-RNA. Immunofluorescence staining and western blotting were performed to assess the relationship between FGF6 and the Hippo pathway. Results We found that FGF6 expression was significantly increased in the MI mouse model. Knockdown of FGF6 synthesis resulted in poorer heart function after MI. By contrast, treatment with recombinant human FGF6 protein improved heart function, reduced infarct size, and promoted cardiac repair. Additionally, FGF6 restrains the activation of the Hippo pathway and subsequently promotes nuclear accumulation of YAP. This was largely counteracted by treatment with extracellular signal-regulated kinase 1/2 (ERK1/2) inhibitor U0126. Conclusion FGF6 inhibits the Hippo pathway via ERK1/2, and facilitates nuclear translocation of YAP, and thereby promotes cardiac repair after MI.
资助项目	National Natural Science Foundation of China [81770498, 81773346, 81870209, 82070507]; Natural Science Foundation of Zhejiang Province [LY21H020009, LZ21H020002, Y19H020042]; Zhejiang Provincial Public Welfare Research Project [LGF20C090002]; Ningbo Public Welfare Research Project [2019C50065]; Basic scientific research project of Wenzhou Key Laboratory [H2020013]; Zhejiang Province Medical and Health Science Program [2019KY099]
出版者	WILEY
出版地	HOBOKEN
ISSN	0960-7722
EISSN	1365-2184
卷号	55 期号:5 页码:e13221
DOI	10.1111/cpr.13221
页数	15
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000775649000001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35355356
SCOPUSEID	2-s2.0-85127446481
通讯作者地址	[Guan, Xueqiang]Department of Cardiology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China ; [Wang, Xu]School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,China ; [Wang, Yang]Department of Histology and Embryology,Institute of Neuroscience,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/147989
专题	第二临床医学院、附属第二医院、育英儿童医院药学院（分析测试中心）附属第二医院基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Wang, Xu; Wang, Yang; Guan, Xueqiang
作者单位	1.Department of Cardiology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 2.School of Pharmaceutical Science,Wenzhou Medical University,Wenzhou,China; 3.Children's Heart Center,Institute of Cardiovascular Development and Translational Medicine,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 4.Department of Histology and Embryology,Institute of Neuroscience,Wenzhou Medical University,Wenzhou,China; 5.College of Pharmacy,Chonnam National University,Gwangju,South Korea; 6.Department of Pharmacy,Ningbo first Hospital,Ningbo,China; 7.School of Medical Technology,Ningbo College of Health Sciences,Ningbo,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 药学院（分析测试中心）
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 药学院（分析测试中心）; 温州医科大学
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Hu, Zhicheng,Chen, Peng,Wang, Linlin,et al. FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway[J]. CELL PROLIFERATION,2022,55(5):e13221.
APA	Hu, Zhicheng., Chen, Peng., Wang, Linlin., Zhu, Yu., Chen, Gen., ... & Guan, Xueqiang. (2022). FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway. CELL PROLIFERATION, 55(5), e13221.
MLA	Hu, Zhicheng,et al."FGF6 promotes cardiac repair after myocardial infarction by inhibiting the Hippo pathway".CELL PROLIFERATION 55.5(2022):e13221.