Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells

doi:10.1016/j.archoralbio.2016.03.009

科研成果详情

题名	Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells
作者	Cen, Sheng-Dan 1; Yu, Wen-Bin2; Ren, Man-Man 1; Chen, Li-Jiao2; Sun, Chao-Fan 2; Ye, Zhi-Li1; Deng, Hui1; Hu, Rong-Dang2
发表日期	2016-08
发表期刊	ARCHIVES OF ORAL BIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Hydrogen sulfide Periodontal ligament cells Osteogenic differentiation Wnt/beta-catenin signaling
其他关键词	OSTEOBLASTIC DIFFERENTIATION ; NITRIC-OXIDE ; STEM-CELLS ; FIBROBLASTS ; DISEASE
摘要	Objective: Endogenous hydrogen sulfide (H2S) has recently emerged as an important intracellular gaseous signaling molecule within cellular systems. Endogenous H2S is synthesized from L-cysteine via cystathionine beta-synthase and cystathionine gamma-lyase and it regulates multiple signaling pathways in mammalian cells. Indeed, aberrant H2S levels have been linked to defects in bone formation in experimental mice. The aim of this study was to examine the potential production mechanism and function of endogenous H2S within primary human periodontal ligament cells (PDLCs). Design: Primary human PDLCs were obtained from donor molars with volunteer permission. Immunofluorescent labeling determined expression of the H2S synthetase enzymes. These enzymes were inhibited with D,L-propargylglycine or hydroxylamine to examine the effects of H2S signaling upon the osteogenic differentiation of PDLCs. Gene and protein expression levels of osteogenic markers in conjunction with ALP staining and activity and alizarin red S staining of calcium deposition were used to assay the progression of osteogenesis under different treatment conditions. Cultures were exposed to Wnt3a treatment to assess downstream signaling mechanisms. Results: In this study, we show that H2S is produced by human PDLC5 via the cystathionine beta-synthase/cystathionine gamma-lyase pathway to promote their osteogenic differentiation. These levels must be carefully maintained as excessive or deficient H2S levels temper the observed osteogenic effect by inhibiting Wnt/beta-catenin signaling. Conclusions: These results demonstrate that optimal concentrations of endogenous H2S must be maintained within PDLCs to promote osteogenic differentiation by activating the Wnt/beta-catenin signaling cascade. (C) 2016 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81200795]; Zhejiang Provincial Nature Science Foundation of China [LY12H14003]; Zhejiang Provincial Medical and Health Science and Technology Plan of China [2015KYA149]; Medical Scientific Research Foundation of Zhejiang Province, China [2015ZA122]
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0003-9969
EISSN	1879-1506
卷号	68 页码:1-8
DOI	10.1016/j.archoralbio.2016.03.009
页数	8
WOS类目	Dentistry, Oral Surgery & Medicine
WOS研究方向	Dentistry, Oral Surgery & Medicine
WOS记录号	WOS:000379369600001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	27035752
SCOPUSEID	2-s2.0-84961942983
通讯作者地址	[Deng, Hui]Department of Periodontics,School of Stomatology,Wenzhou Medical University,No 113 West Xueyuan Road,Wenzhou, Zhejiang,China
Scopus学科分类	Otorhinolaryngology;Dentistry (all);Cell Biology
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14722
专题	口腔医学院、附属口腔医院_口腔正畸
通讯作者	Deng, Hui
作者单位	1.Department of Periodontics,School of Stomatology,Wenzhou Medical University,No 113 West Xueyuan Road,Wenzhou, Zhejiang,China; 2.Department of Orthodontics,School of Stomatology,Wenzhou Medical University,No 113 West Xueyuan Road,Wenzhou, Zhejiang,China
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Cen, Sheng-Dan,Yu, Wen-Bin,Ren, Man-Man,et al. Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells[J]. ARCHIVES OF ORAL BIOLOGY,2016,68:1-8.
APA	Cen, Sheng-Dan., Yu, Wen-Bin., Ren, Man-Man., Chen, Li-Jiao., Sun, Chao-Fan., ... & Hu, Rong-Dang. (2016). Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells. ARCHIVES OF ORAL BIOLOGY, 68, 1-8.
MLA	Cen, Sheng-Dan,et al."Endogenous hydrogen sulfide is involved in osteogenic differentiation in human periodontal ligament cells".ARCHIVES OF ORAL BIOLOGY 68(2016):1-8.