HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis

doi:10.3892/or.2017.5893

科研成果详情

题名	HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis
作者	Song, Xiaoxiao 1; Liao, Zhisu1; Zhou, Chunchun1; Lin, Renyu1; Lu, Jianglong2; Cai, Lin2; Tan, Xiaohua3; Zeng, Wenjian 1; Lu, Xianghe2; Zheng, Weiming2; Chen, Jianfu1; Su, Zhipeng2
发表日期	2017-10
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	HSP47 laryngeal squamous cell carcinoma prognosis cisplatin proliferation invasion apoptosis
其他关键词	HEAT-SHOCK PROTEINS ; BREAST-CANCER ; FOLLOW-UP ; EXPRESSION ; CHEMOTHERAPY ; NECK ; CHAPERONE ; SURVIVAL ; OVEREXPRESSION ; STATISTICS
摘要	Heat shock protein 47 (HSP47) is a 47 kDa collagen binding protein that has a close relationship with the development and progression of tumours. However, little is known concerning the expression profile of HSP47 in laryngeal squamous cell carcinoma (LSCC) patients and there is still insufficient data concerning the underlying mechanisms. The aim of the present study was to explore the expression of HSP47 in LSCC and provide an overview of its association with tumourigenicity and clinical prognosis. The expression of HSP47 in LSCC and adjacent non-cancerous laryngeal tissues was assessed via western blotting and immunohistochemical studies. The prognostic significance of HSP47 expression was analysed using a Kaplan-Meier survival curve. To investigate the influence of HSP47 on the viability, invasion and apoptosis of a LSCC cell line, we performed an in vitro analysis with plasmid vectors and small interfering RNA (siRNA). Our results showed that HSP47 protein expression in the LSCC tissues was markedly decreased compared to that noted in the adjacent non-cancerous tissues, and low expression of HSP47 was correlated with poor prognosis in LSCC patients. Upregulation of HSP47 via plasmid vectors inhibited the proliferation, reduced the invasive ability, increased the sensitivity to cisplatin chemotherapy, promoted apoptosis, and induced the G1 phase arrest of LSCC cells in vitro. The expression of apoptosis-regulating proteins was also altered when HSP47 was upregulated, involving increased expression of cleaved caspase-7/-8/-9, PARP, and Bax and decreased expression of Bcl-2. Our present data suggest that HSP47 is an important prognostic factor and an attractive therapeutic target in LSCC due to its influence on the biological behaviour of LSCC cells.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81201687]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H130003, LY16H160051, LY13H090009]; Zhejiang Province Medical and Health Science and Technology Plan Project [2012RCA042]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	38 期号:4 页码:2444-2452
DOI	10.3892/or.2017.5893
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000411688200063
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28849239
SCOPUSEID	2-s2.0-85029817632
通讯作者地址	[Su, Zhipeng]Departments of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University,3 Fuxue Road,Wenzhou, Zhejiang,325000,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14700
专题	附属第一医院药学院（分析测试中心）
通讯作者	Su, Zhipeng
作者单位	1.Departments of Otolaryngology,First Affiliated Hospital of Wenzhou Medical University,3 Fuxue Road,Wenzhou, Zhejiang,325000,China; 2.Departments of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University,3 Fuxue Road,Wenzhou, Zhejiang,325000,China; 3.Key Laboratory of Biotechnology and Pharmaceutical Engineering,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou, Zhejiang,325000,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Song, Xiaoxiao,Liao, Zhisu,Zhou, Chunchun,et al. HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis[J]. ONCOLOGY REPORTS,2017,38(4):2444-2452.
APA	Song, Xiaoxiao., Liao, Zhisu., Zhou, Chunchun., Lin, Renyu., Lu, Jianglong., ... & Su, Zhipeng. (2017). HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis. ONCOLOGY REPORTS, 38(4), 2444-2452.
MLA	Song, Xiaoxiao,et al."HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by inhibiting cell viability and invasion and promoting apoptosis".ONCOLOGY REPORTS 38.4(2017):2444-2452.