Determination of Oxycodone in Rat Plasma by a Simple Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

科研成果详情

题名	Determination of Oxycodone in Rat Plasma by a Simple Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
作者	Liu, Fuli1; Ye, Luxin2; Dong, Yanwen 2; Wang, Qianqian 2; Cai, Fuman3
发表日期	2018-12-31
发表期刊	LATIN AMERICAN JOURNAL OF PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	oxycodone pharmacokinetics plasma UPLC-MS/MS
其他关键词	UPLC-MS/MS ; NOROXYCODONE ; OXYMORPHONE ; VALIDATION ; PHARMACOKINETICS ; QUANTITATION ; TISSUE
摘要	The aim of this study is to develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine oxycodone in rat plasma. Diazepam was used as internal standard (IS). Rat plasma samples were processed by acetonitrile precipitation. Chromatographic separation was conducted on a UPLC BEH C18 column. The mobile phase consisted of acetonitrile and 0.1% formic acid, and the gradient elution mode was used with the total running time of 4.0 min. Electrospray ionization (ESI) source was selected. Under the positive ion mode, the quantification was performed by multiple reactions monitoring (MRM) with transition of m/z 316.1 -> 298.1 for oxycodone and m/z 285.1 -> 193.3 for IS. The results showed that in the range of 0.2-100 ng/mL, oxycodone was linear in rat plasma (r > 0.995) with the lower limit of quantification (LLOQ) of 0.2 ng/mL. The intra-day and interday precision represented as RSD of oxycodone was less than 11 and 12%, respectively. The accuracy was between 88.4 and 109.9%. The recovery was higher than 68.3% and the matrix effect was between 97.2 and 106.0%. The method is sensitive, rapid and selective and has been successfully applied to the pharmacokinetics of oxycodone in rats.
资助项目	Wenzhou Science and Technology Bureau [Y20170651, Y20150173, Y20160187]
出版者	COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES
出版地	LA PLATA
ISSN	0326-2383
卷号	37 期号:3 页码:611-615
页数	5
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000429543900035
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85045128702
通讯作者地址	[Liu, Fuli]Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Pharmaceutical Science
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14694
专题	附属第一医院实验动物中心
通讯作者	Liu, Fuli
作者单位	1.Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Laboratory Animal Centre,Wenzhou Medical University,Wenzhou,China; 3.Department of Nursing,Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liu, Fuli,Ye, Luxin,Dong, Yanwen,et al. Determination of Oxycodone in Rat Plasma by a Simple Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2018,37(3):611-615.
APA	Liu, Fuli, Ye, Luxin, Dong, Yanwen, Wang, Qianqian, & Cai, Fuman. (2018). Determination of Oxycodone in Rat Plasma by a Simple Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. LATIN AMERICAN JOURNAL OF PHARMACY, 37(3), 611-615.
MLA	Liu, Fuli,et al."Determination of Oxycodone in Rat Plasma by a Simple Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry".LATIN AMERICAN JOURNAL OF PHARMACY 37.3(2018):611-615.