Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats

doi:10.1111/jcmm.15886

科研成果详情

题名	Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats
作者	Yu, Yige 1; Li, Zengqiang 2; Ma, Feifei 2; Chen, Quanxu2; Lin, Liben 2; Xu, Qiang 2; Li, Yang 2; Xin, Xiu 2; Pan, Peipei 1; Huang, Tongliang1; Wang, Yiyan1; Fei, Qianjin3; Ge, Ren-Shan1,2
发表日期	2020-12
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	differentiation NT‐ 3 proliferation stem Leydig cell Trkc
其他关键词	ETHANE DIMETHYL SULFONATE ; EMBRYONIC TESTIS ; DIFFERENTIATION ; MOUSE ; GENE ; MICE ; DESTRUCTION ; ACTIVATION ; PRECURSORS ; EXPRESSION
摘要	Neurotrophin-3 (NT-3) acts as an important growth factor to stimulate and control tissue development. The NT-3 receptor, TRKC, is expressed in rat testis. Its function in regulation of stem Leydig cell development and its underlying mechanism remain unknown. Here, we reported the role of NT-3 to regulate stem Leydig cell development in vivo and in vitro. Ethane dimethane sulphonate was used to kill all Leydig cells in adult testis, and NT-3 (10 and 100 ng/testis) was injected intratesticularly from the 14th day after ethane dimethane sulphonate injection for 14 days. NT-3 significantly reduced serum testosterone levels at doses of 10 and 100 ng/testis without affecting serum luteinizing hormone and follicle-stimulating hormone levels. NT-3 increased CYP11A1-positive Leydig cell number at 100 ng/testis and lowered Leydig cell size and cytoplasmic size at doses of 10 and 100 ng/testis. After adjustment by the Leydig cell number, NT-3 significantly down-regulated the expression of Leydig cell genes (Lhcgr, Scarb1, Star, Cyp11a1, Hsd3b1, Cyp17a1, Hsd17b3, Hsd11b1, Insl3, Trkc and Nr5a1) and the proteins. NT-3 increased the phosphorylation of AKT1 and mTOR, decreased the phosphorylation of 4EBP, thereby increasing ATP5O. In vitro study showed that NT-3 dose-dependently stimulated EdU incorporation into stem Leydig cells and inhibited stem Leydig cell differentiation into Leydig cells, thus leading to lower medium testosterone levels and lower expression of Lhcgr, Scarb1, Trkc and Nr5a1 and their protein levels. NT-3 antagonist Celitinib can antagonize NT-3 action in vitro. In conclusion, the present study demonstrates that NT-3 stimulates stem Leydig cell proliferation but blocks the differentiation via TRKC receptor.
资助项目	Wenzhou Municipal Science and Technology Bureau [ZS2017009]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81601266, 81730042]; Science and Technology Department of Zhejiang Province [2019C03035]
出版者	WILEY
出版地	HOBOKEN
ISSN	1582-1838
EISSN	1582-4934
卷号	24 期号:23 页码:13679-13689
DOI	10.1111/jcmm.15886
页数	11
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:000582297700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33090725
PMC记录号	PMC7753877
SCOPUSEID	2-s2.0-85092923271
通讯作者地址	[Ge, Ren-Shan]Department of Obstetrics and Gynecology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China ; [Ge, Ren-Shan]Department of Anesthesiology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China ; [Fei, Qianjin]Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Molecular Medicine;Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14639
专题	第二临床医学院、附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院附属第二医院附属第一医院附属第一医院_生殖医学中心附属第二医院_妇产科
通讯作者	Fei, Qianjin; Ge, Ren-Shan
作者单位	1.Department of Obstetrics and Gynecology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Anesthesiology,The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Wenzhou,China; 3.Reproductive Medicine Center,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科; 附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 生殖医学中心
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Yu, Yige,Li, Zengqiang,Ma, Feifei,et al. Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020,24(23):13679-13689.
APA	Yu, Yige., Li, Zengqiang., Ma, Feifei., Chen, Quanxu., Lin, Liben., ... & Ge, Ren-Shan. (2020). Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 24(23), 13679-13689.
MLA	Yu, Yige,et al."Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 24.23(2020):13679-13689.