Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography

doi:10.1007/s10753-015-0119-z

科研成果详情

题名	Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography
作者	Wang, Yuanyuan 1; Zhao, Jing 1; Tan, Lifei1; Huang, Yueyue2; Li, Dequan3; Quan, Shichao4; Li, Min 1; Pan, Jingye2
发表日期	2015-08
发表期刊	INFLAMMATION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	bone marrow mesenchymal stem cells extracellular kynurenine cell therapeutics high-performance liquid chromatography
其他关键词	INDOLEAMINE 2,3-DIOXYGENASE ACTIVITY ; INTERFERON-GAMMA ; INFLAMMATION ; SEPSIS ; PLASMA ; ASSOCIATION ; PATHWAY ; INJURY
摘要	Endothelial cell dysfunction plays an important role in the occurrence and development of sepsis, which is a consequence of the interaction between coagulation and inflammation. Kynurenine (KYN) is an endothelium-derived relaxing factor that makes a large contribution to sepsis pathophysiology. In this study, we investigated the influence of bone marrow mesenchymal stem cells (BMSCs) on KYN production by cultured endothelial cells. KYN and tryptophan (TRP) concentrations in cell supernatants were simultaneously measured with a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector (FLD) and an ultraviolet detector (UVD). Our results revealed that lipopolysaccharide-stimulated endothelial cells produced more KYN, which was accompanied by a parallel decrease in TRP. When co-cultured with BMSCs, KYN and TRP production were significantly decreased compared to lipopolysaccharide (LPS)-induction alone. Our results suggest that BMSCs can attenuate endothelial cell damage by decreasing KYN as detected with HPLC. This method is the first to be capable of capturing functional changes in the cells and is simple, fast, and suitable for cellular level research purposes.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81171787]; Zhejiang Province Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY12H15001]
出版者	SPRINGER/PLENUM PUBLISHERS
出版地	NEW YORK
ISSN	0360-3997
EISSN	1573-2576
卷号	38 期号:4 页码:1450-1457
DOI	10.1007/s10753-015-0119-z
页数	8
WOS类目	Cell Biology ; Immunology
WOS研究方向	Cell Biology ; Immunology
WOS记录号	WOS:000357666000008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25854176
SCOPUSEID	2-s2.0-84937252281
通讯作者地址	[Pan, Jingye]Department of Intensive Care Unit,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1462
专题	附属第一医院附属第一医院_重症医学科（ICU）
通讯作者	Pan, Jingye
作者单位	1.Department of the First Clinical Medical,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Intensive Care Unit,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Traumatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 4.Department of General Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wang, Yuanyuan,Zhao, Jing,Tan, Lifei,et al. Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography[J]. INFLAMMATION,2015,38(4):1450-1457.
APA	Wang, Yuanyuan., Zhao, Jing., Tan, Lifei., Huang, Yueyue., Li, Dequan., ... & Pan, Jingye. (2015). Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography. INFLAMMATION, 38(4), 1450-1457.
MLA	Wang, Yuanyuan,et al."Bone Marrow Mesenchymal Stem Cells Alleviate Extracellular Kynurenine Levels, as Detected by High-Performance Liquid Chromatography".INFLAMMATION 38.4(2015):1450-1457.