Determination of bupropion and its main metabolite in rat plasma by LC-MS and its application to pharmacokinetics

doi:10.1691/ph.2011.1078

科研成果详情

题名	Determination of bupropion and its main metabolite in rat plasma by LC-MS and its application to pharmacokinetics
作者	Hu, Lufeng1; Wang, Zhe2; Xu, Renai1; Ma, Jianshe3; Wang, Xianqin2; Zhang, Xiuhua1
发表日期	2011-12
发表期刊	PHARMAZIE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	HUMAN CYTOCHROME P4502B6 ; LIQUID-CHROMATOGRAPHY ; DRUG-INTERACTIONS ; IN-VITRO ; HYDROXYLATION ; INHIBITION ; CYP2B6
摘要	A sensitive and selective liquid chromatograpy-mass spectrometry method for the determination of bupropion and its main metabolite, hydroxubupropion, in rat plasma was developed and validated. After addition of carbamazepine as internal standard (IS) and precipitation of protein with acetonitrile, the plasma samples were analyzed on an Agilent Zorbax SB-C(18) (2.1 mm x 50 mm, 3.5 mu m) column at 30 degrees C, with acetonitrile-0.1% formic acid as mobile phase at a flow rate of 0.4 mL min(-1). The detection was carried out in the selective ion monitoring mode with a positive electrospray ionization interface. The calibration curve was linear over the 10-2000 ng mL(-1) for bupropion and 5-1000 ng mL(-1) for hydroxybupropion in plasma. RSD of inter-day and intra-day precision was less than 7% for bupropion, 9% for hydroxybupropion. The developed method was successfully applied to pharmacokinetic studies after single intragastric administration of bupropion 15 mg kg(-1) to rats.
资助项目	Wenzhou Municipal Science and Technology projects [Y20090048, Y20090143]
出版者	GOVI-VERLAG PHARMAZEUTISCHER VERLAG GMBH
出版地	ESCHBORN
ISSN	0031-7144
卷号	66 期号:12 页码:924-928
DOI	10.1691/ph.2011.1078
页数	5
WOS类目	Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy ; Chemistry
WOS记录号	WOS:000298937100004
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22312695
SCOPUSEID	2-s2.0-84862964613
通讯作者地址	[Zhang, Xiuhua]Wenzhou Med Coll, Affiliated Hosp 1, Dept Pharm, Wenzhou 325000, Peoples R China.
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14594
专题	附属第一医院_药学部药学部药学院（分析测试中心）基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Zhang, Xiuhua
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Pharm, Wenzhou 325000, Peoples R China; 2.Wenzhou Med Coll, Dept Pharmacol, Wenzhou 325000, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325000, Peoples R China
第一作者单位	附属第一医院; 药学部药学部
通讯作者单位	附属第一医院; 药学部药学部
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Lufeng,Wang, Zhe,Xu, Renai,et al. Determination of bupropion and its main metabolite in rat plasma by LC-MS and its application to pharmacokinetics[J]. PHARMAZIE,2011,66(12):924-928.
APA	Hu, Lufeng, Wang, Zhe, Xu, Renai, Ma, Jianshe, Wang, Xianqin, & Zhang, Xiuhua. (2011). Determination of bupropion and its main metabolite in rat plasma by LC-MS and its application to pharmacokinetics. PHARMAZIE, 66(12), 924-928.
MLA	Hu, Lufeng,et al."Determination of bupropion and its main metabolite in rat plasma by LC-MS and its application to pharmacokinetics".PHARMAZIE 66.12(2011):924-928.