CD4(+) T cell proliferation and inhibition of activation-induced cell death (AICD) in childhood asthma

科研成果详情

题名	CD4(+) T cell proliferation and inhibition of activation-induced cell death (AICD) in childhood asthma
作者	Lu, Huajun ; Fang, Lingjuan ; Lin, Xiaochun ; Li, Xiaoli ; Huang, Kaiyu ; Yan, Xiumei 1,2
发表日期	2018-12-31
发表期刊	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Childhood asthma proliferation Th cells activation-induced cell death
其他关键词	APOPTOSIS ; INFLAMMATION ; LYMPHOCYTES ; CYTOKINES
摘要	Objective: The mechanism underlying immune inflammation and over-activation of T helper (Th) cells in childhood asthma was investigated through cell proliferation and activation-induced cell death (AICD) experiments. Methods: There were 30 children in the asthma group with an average age of 10.2 +/- 3.1 years and 30 children in the normal control group with an average age of 10.5 +/- 2.9 years. A cytometric bead array (CBA) was performed to detect Th1, Th2, and Th17 cytokines. After CD4(+) T cells were separated using immunomagnetic beads and stimulated by phytohemagglutinin (PHA) combined with anti-CD3 antibodies in vitro, cell proliferation and AICD were analyzed. Finally, mRNA expression of the apoptosis-and proliferation-related proteins Fas, FasL, and Bcl-2 was detected using quantitative polymerase chain reaction (PCR). Results: The serum cytokine levels of the children in the asthma group significantly increased compared to those of children in the normal control group (IL-4: 9.33 +/- 2.25 ng/L vs. 4.59 +/- 1.77 ng/L, P = 0.012; IL-10: 3.41 +/- 0.80 ng/L vs. 1.36 +/- 0.41 ng/L, P = 0.027; TNF: 7.63 +/- 4.09 ng/L vs. 1.88 +/- 0.52 ng/L, P = 0.023). The CD4(+) T cell proliferation ability in the asthma group was significantly higher than that in the normal control group (OD450: 0.66 +/- 0.14 vs. 0.28 +/- 0.07, P<0.001), whereas the AICD rate was significantly lower than that in the normal control group (29.46 +/- 5.25% vs. 60.11 +/- 4.93%, P<0.001). The Fas mRNA expression in the CD4(+) T cells from children in the asthma group was significantly decreased compared to that from the children in the normal control group, whereas the Bcl-2 expression was significantly higher than that from children in the normal control group. These differences were both statistically significant (P<0.001). Conversely, FasL expression did not differ (P > 0.05). Conclusion: Fas expression decreased and Bcl-2 expression increased in CD4(+) T cells from the children in the asthma group, inhibiting AICD, and promoting proliferation of Th cells to some extent. Apoptosis inhibition and cell proliferation might result in over-activation of Th cells and aggravation of inflammatory infiltration in children with asthma.
出版者	E-CENTURY PUBLISHING CORP
出版地	MADISON
ISSN	1940-5901
卷号	11 期号:3 页码:2319-2324
页数	6
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000429006600104
收录类别	SCIE
通讯作者地址	[Yan, Xiumei]Wenzhou Med Univ, Dept Pediat Gastroenterol, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China. ; [Yan, Xiumei]Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China.
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14522
专题	附属第二医院_儿童消化科第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Yan, Xiumei
作者单位	1.Wenzhou Med Univ, Dept Pediat Gastroenterol, Affiliated Hosp 2, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuan West Rd, Wenzhou 325027, Zhejiang, Peoples R China
通讯作者单位	附属第二医院_儿童消化科; 第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Lu, Huajun,Fang, Lingjuan,Lin, Xiaochun,et al. CD4(+) T cell proliferation and inhibition of activation-induced cell death (AICD) in childhood asthma[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2018,11(3):2319-2324.
APA	Lu, Huajun, Fang, Lingjuan, Lin, Xiaochun, Li, Xiaoli, Huang, Kaiyu, & Yan, Xiumei. (2018). CD4(+) T cell proliferation and inhibition of activation-induced cell death (AICD) in childhood asthma. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 11(3), 2319-2324.
MLA	Lu, Huajun,et al."CD4(+) T cell proliferation and inhibition of activation-induced cell death (AICD) in childhood asthma".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 11.3(2018):2319-2324.