AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14

doi:10.3389/fmicb.2019.01847

科研成果详情

题名	AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14
作者	Hou, Lingli1,2; Debru, Alexander 1; Chen, Qianqian 1; Bao, Qiyu1; Li, Kewei1
发表日期	2019-08-14
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Pseudomonas aeruginosa AmrZ swarming motility cyclic di-GMP GcbA FIgZ PA14_56180 exopolysaccharide
其他关键词	BIOFILM FORMATION ; PILZ DOMAIN ; VI SECRETION ; PROTEIN ; ALGZ ; BIOSYNTHESIS ; REPRESSION ; EXPRESSION ; REQUIRES ; BACTERIA
摘要	Swarming is a surface-associated motile behavior that plays an important role in the rapid spread, colonization, and subsequent establishment of bacterial communities. In Pseudomonas aeruginosa, swarming is dependent upon a functional flagella and aided by the production of biosurfactants. AmrZ, a conserved transcription factor across pseudomonads, has been shown to be a global regulator of multiple genes important for virulence and ecological fitness. In this study, we expand this concept of global control to swarming motility by showing that deletion of amrZ results in a severe defect in swarming, while multicopy expression of this gene stimulates swarming of P aeruginosa. Mechanistic studies showed that the swarming defect of an amrZ mutant does not involve changes of biosurfactant production but is associated with flagellar malfunction. The Delta amrZ mutant exhibits increased levels of the second messenger cyclic di-GMP (c-di-GMP) compared to the wild-type strain, under swarming conditions. We found that the diguanylate cyclase GcbA was the main contributor to the increased accumulation of c-di-GMP observed in the Delta amrZ mutant and was a strong inhibitor of flagellar-dependent motility. Our results revealed that the GcbA-dependent inhibition of motility required the presence of two c-di-GMP receptors containing a PilZ domain: FIgZ and PA14_56180. Furthermore, the Delta amrZ mutant exhibits enhanced production of Pel polysaccharide. Epistasis analysis revealed that GcbA and the Pel polysaccharide act independently to limit swarming in Delta amrZ. Our results support a role for AmrZ in controlling swarming motility, yet another social behavior besides biofilm formation that is crucial for the ability of P aeruginosa to colonize a variety of surfaces. The central role of AmrZ in controlling these behaviors makes it a good target for the development of treatments directed to combat P. aeruginosa infections.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31500109, 81870075]; Natural Science Foundation for Youths of Zhejiang Province [LQ16C010001]; China Scholarship Council (CSC) GrantChina Scholarship Council [201708330005]; Wenzhou Medical University [QTJ14024]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-302X
卷号	10 页码:1847
DOI	10.3389/fmicb.2019.01847
页数	16
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000480706700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31474950
PMC记录号	PMC6707383
SCOPUSEID	2-s2.0-85078986024
通讯作者地址	[Bao, Qiyu]Department of Microbiology and Immunology,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China ; [Li, Kewei]Department of Microbiology and Immunology,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Microbiology;Microbiology (medical)
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14487
专题	检验医学院（生命科学学院、生物学实验教学中心）附属第二医院_科研中心
通讯作者	Bao, Qiyu; Li, Kewei
作者单位	1.Department of Microbiology and Immunology,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 2.Scientific Research Center,Wenzhou Medical University,Wenzhou,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 温州医科大学
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Hou, Lingli,Debru, Alexander,Chen, Qianqian,et al. AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14[J]. FRONTIERS IN MICROBIOLOGY,2019,10:1847.
APA	Hou, Lingli, Debru, Alexander, Chen, Qianqian, Bao, Qiyu, & Li, Kewei. (2019). AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14. FRONTIERS IN MICROBIOLOGY, 10, 1847.
MLA	Hou, Lingli,et al."AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production in Pseudomonas aeruginosa PA14".FRONTIERS IN MICROBIOLOGY 10(2019):1847.