Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma

doi:10.1007/s00253-021-11559-6

科研成果详情

题名	Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma
作者	Kamara, Saidu; Guo, Yanru; Mao, Shanshan; Ye, Xiaoxian; Li, Qingfeng; Zheng, Maolin; Zhu, Jinshun; Zhang, Jing; Du, Wangqi; Chen, Jun; Zhu, Shanli; Zhang, Lifang
发表日期	2021-10
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Affibody molecules EBV LMP1 Nasopharyngeal carcinoma Molecular imaging
其他关键词	EPSTEIN-BARR-VIRUS ; EXPRESSION ; PROTEINS
摘要	Nasopharyngeal carcinoma (NPC) is consistently associated with Epstein-Barr virus (EBV) latent infection and is common in Southern China and Southeast Asia. The viral latent membrane proteins LMP1 and LMP2 are persistently expressed in NPC tissues; the cytoplasmic domain of LMP1 (LMP1 C-terminal) and LMP2A (LMP2A N-terminal) proteins is essential for maintenance of latency and can alter host cell signaling to facilitate tumor growth and progression. Thus, targeting LMP1 or LMP2 oncoprotein has been an increasing interest for diagnosis and targeted therapy of NPC. Affibody molecules, a new class of small-affinity engineered scaffold proteins, have demonstrated high potential for therapeutics, diagnostics, and biotechnological applications. More recently, radiolabelled HER2-specific affibody molecules have demonstrated to be useful in imaging of HER2 expressing tumor. In this study, we report three novel EBV LMP1 C-terminal (EBV LMP1-C) domain affibody molecules (Z(LMP1-C)15, Z(LMP1-C)114, and Z(LMP1-C)277) were selected by biopanning from a random-peptide displayed phage library and used for molecular imaging in tumor-bearing nude mice. Surface plasmon resonance (SPR), indirect immunofluorescence, and immunohistochemistry (IHC) clearly showed that all three selected affibody molecules have high affinity and specificity in binding to EBV LMP1 protein. Moreover, in vivo tumor imaging revealed that Dylight-755-labeled affibody molecules accumulated rapidly in tumor site after injection (1 h) and then were continuously maintained for 24 h in EBV-positive NPC xenograft mice model. In conclusion, our findings highlight the potential use of Z(LMP1-C) affibody molecules as tumor-specific molecular imaging agents of EBV-associated NPC.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81972550, 81372447]
出版者	SPRINGER
出版地	NEW YORK
ISSN	0175-7598
EISSN	1432-0614
卷号	105 期号:19 页码:7283-7293
DOI	10.1007/s00253-021-11559-6
页数	36
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
WOS记录号	WOS:000694774600002
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20213710888330
EI主题词	Molecular imaging
URL	查看原文
PubMed ID	34505914
SCOPUSEID	2-s2.0-85114686875
通讯作者地址	[Zhang, Lifang]Institute of Molecular Virology and Immunology,Department of Microbiology and Immunology,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Biotechnology;Applied Microbiology and Biotechnology
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14443
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, Lifang
作者单位	Institute of Molecular Virology and Immunology,Department of Microbiology and Immunology,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
第一作者的第一单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系
推荐引用方式 GB/T 7714	Kamara, Saidu,Guo, Yanru,Mao, Shanshan,et al. Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2021,105(19):7283-7293.
APA	Kamara, Saidu., Guo, Yanru., Mao, Shanshan., Ye, Xiaoxian., Li, Qingfeng., ... & Zhang, Lifang. (2021). Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 105(19), 7283-7293.
MLA	Kamara, Saidu,et al."Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 105.19(2021):7283-7293.