Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells

doi:10.12659/MSM.903892

科研成果详情

题名	Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells
作者	Yu, Yang 1; Lin, Lixiang 1; Zhou, Yifei1; Lu, Xiaolang1; Shao, Xiwen 1; Lin, Chuanlu 1; Yu, Kehe1; Zhang, Xiaolei 1; Hong, Jianjun1; Chen, Ying 2
发表日期	2017-03-17
发表期刊	MEDICAL SCIENCE MONITOR 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Adult Stem Cells Antigens Differentiation Cell Hypoxia
其他关键词	REDUCED OXYGEN-TENSION ; OSTEOGENIC DIFFERENTIATION ; MITOCHONDRIAL BIOGENESIS ; EXTRACELLULAR-MATRIX ; ANTIOXIDANT ENZYMES ; DEFECT MODEL ; ROTATOR CUFF ; IN-VITRO ; REPAIR ; TENOCYTES
摘要	Background: Hypoxic conditions play roles in functioning of human tendon-derived stem cells (hTSCs). The goal of this study was to investigate the effect of various hypoxic conditions in self-renewal capacity and differentiation of TSCs. Material/Methods: hTSCs was obtain from supraspinatus tendon donors. Colony formation and cell proliferation assay were used to assess the self-renewal of hTSCs. qRT-PCT and Western blot analysis were used to examine stemness and multi-differentiation potential of hTSCs. Results: We found that culturing at 5% O-2 is more beneficial for the self-renewal of hTSCs than the other 3 culture conditions, with larger colony size and numbers. The proliferation of hTSCs in 5%, 10%, and 20% O-2 cultures increased after seeding. The number of cells in the 5% O-2 condition was higher than that in other culture; however, self-renewal capacity of hTSCs in 0.5% O-2 was inhibited. The expression levels of stem cell markers, including NS, Nanog, Oct-4, and SSEA-4, were highest in 0.5% O-2 culture. Furthermore, hTSCs cultured in 20% O-2 exhibited significantly higher expression of the 3 markers (PPAR-g, Sox-9, and Runx-2). Conclusions: Hypoxic condition of culture encouraged self-renewal capacity of hTSCs, but inhibited their multi-differentiation potential, compared to normoxic condition of culture. Moreover, excessively low oxygen concentration impaired the capacity of hTSCs.
出版者	INT SCIENTIFIC LITERATURE, INC
出版地	SMITHTOWN
ISSN	1643-3750
EISSN	1643-3750
卷号	23 页码:1334-1339
DOI	10.12659/MSM.903892
页数	6
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000396981600001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	28302994
PMC记录号	PMC5367841
SCOPUSEID	2-s2.0-85015894517
通讯作者地址	[Hong, Jianjun]Department of Orthopaedic Surgery,The 2nd Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Medicine (all)
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14358
专题	第二临床医学院、附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院_急诊医学
通讯作者	Hong, Jianjun
作者单位	1.Department of Orthopaedic Surgery,The 2nd Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Emergency Medicine,The 2nd Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Yu, Yang,Lin, Lixiang,Zhou, Yifei,et al. Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells[J]. MEDICAL SCIENCE MONITOR,2017,23:1334-1339.
APA	Yu, Yang., Lin, Lixiang., Zhou, Yifei., Lu, Xiaolang., Shao, Xiwen., ... & Chen, Ying. (2017). Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells. MEDICAL SCIENCE MONITOR, 23, 1334-1339.
MLA	Yu, Yang,et al."Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells".MEDICAL SCIENCE MONITOR 23(2017):1334-1339.