Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus

doi:10.2147/DMSO.S160301

科研成果详情

题名	Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus
作者	Yang, Gaoxiong 1; Yan, Rui 2; Tong, Huanjun 3; Zhang, Jitai 1; Chen, Bin 4; Xue, Xiangyang5; Wang, Jue6; Chu, Maoping7; Jin, Shengwei8; Li, Ming 1
发表日期	2018-12-31
发表期刊	DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	neutrophil-lymphocyte ratio diabetes oscillating glucose level innate immune rat
其他关键词	OXIDATIVE STRESS ; ACTIVATION ; APOPTOSIS ; ALLOXAN ; BLOOD ; PREDICTOR ; ANALYZER ; MODELS ; KIDNEY ; CELLS
摘要	Background: The neutrophil-lymphocyte ratio (NLR) has been considered as an inflammatory marker in various disorders, but it is not clear whether the NLR is also elevated with hidden diabetes (HD), which is normal in fasting blood glucose (FBG) but abnormal in the oral glucose tolerance test (OGTT). Materials and methods: An HD animal model for 27 days and an animal model with oscillating glucose (OG) for 7 days were applied on adult female Sprague-Dawley rats. OGTT, leukogram analysis, histology, and immunohistochemistry were carried out. Results: In HD rats, the percentage of neutrophils increased but the percentage of lymphocytes decreased; hence, the NLR rose relative to sham. This may be a result of the OG levels often experienced by diabetic subjects, as normal rats given OG (6 g/kg/6 h) for 7 days had significantly reduced lymphocyte numbers and increased NLR compared with the values before and 1 h after oral glucose administration during OGTT. Glucose-induced disarrangement of partitions of circulating immune cells and NLR was involved in the increase in oxidative stress, as these changes were totally blocked by the antioxidant glutathione (GSH). GSH (50 mg/kg/6 h) totally blocked the glucose-induced alterations in lymphocyte and NLR values. Conclusion: HD associated with elevation of NLR values may be partly attributed to a homeostasis disorder of the innate inflammatory state, caused by oscillating hyperglycemia. Acute high glucose administration produced a significant decrease in lymphocyte number. OG administration potentiated this effect and increased the NLR value, which was blocked by GSH, suggesting that reactive oxygen species play a critical role in maintaining lymphocyte numbers.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670336]; Wenzhou Grant for Scientific Talents, Wenzhou Science and Technology Bureau [RX2016003]
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1178-7007
卷号	11 页码:277-288
DOI	10.2147/DMSO.S160301
页数	12
WOS类目	Endocrinology & Metabolism
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:000435095100001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29942142
PMC记录号	PMC6005307
SCOPUSEID	2-s2.0-85057019067
通讯作者地址	[Li, Ming]Cardiac Regeneration Research Institute,Wenzhou Medical University,Chashan Higher Education Park,Wenzhou,325035,China
Scopus学科分类	Internal Medicine;Pharmacology
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1430
专题	基础医学院（机能实验教学中心）_基础医学研究中心_心脏再生医学研究所检验医学院（生命科学学院、生物学实验教学中心）附属第二医院附属第一医院第二临床医学院、附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Li, Ming
作者单位	1.Cardiac Regeneration Research Institute,Wenzhou Medical University,Wenzhou,China; 2.School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou,China; 3.The Second Clinical Medical College,Wenzhou Medical University,Wenzhou,China; 4.Department of Medical Ultrasound,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 5.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of Tropical Medicine,Wenzhou Medical University,Wenzhou,China; 6.Department of Cardiac Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 7.Children’s Heart Center,The Second Affiliated Hospital and Yuying Children’s Hospital,Institute of Cardiovascular Development and Translational Medicine,Wenzhou Medical University,Wenzhou,China; 8.Department of Anesthesia and Critical Care,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Yang, Gaoxiong,Yan, Rui,Tong, Huanjun,et al. Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus[J]. DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY,2018,11:277-288.
APA	Yang, Gaoxiong., Yan, Rui., Tong, Huanjun., Zhang, Jitai., Chen, Bin., ... & Li, Ming. (2018). Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus. DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY, 11, 277-288.
MLA	Yang, Gaoxiong,et al."Chronic oscillating glucose challenges disarrange innate immune homeostasis to potentiate the variation of neutrophil-lymphocyte ratio in rats with or without hidden diabetes mellitus".DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY 11(2018):277-288.