Determination of Rhynchophylline in Rat Plasma by Liquid Chromatography Mass Spectrometry and Its Application

doi:10.1093/chromsci/bmt096

科研成果详情

题名	Determination of Rhynchophylline in Rat Plasma by Liquid Chromatography Mass Spectrometry and Its Application
作者	Cai, Jinzhang1; Lin, Chongliang2; Ma, Jianshe3; Hu, Lufeng2; Lin, Guanyang2; Wang, Xianqin4
发表日期	2014-08
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	LC-MS-MS ; RABBIT PLASMA ; UNCARIA-RHYNCHOPHYLLA ; HYDROCHLORIDE ; ALKALOIDS ; LEAVES
摘要	A sensitive and selective liquid chromatography mass spectrometry method was developed for the determination of rhynchophylline in rat plasma. After the addition of estazolam as the internal standard (IS), protein precipitation by acetonitrile was used for sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 column (2.1 x 150 mm, 5 mu m) with acetonitrile-0.1% formic acid as the mobile phase with gradient elution. The electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification by using target fragment ions m/z 385 for rhynchophylline and m/z 295 for the IS. Calibration plots were linear over the range of 5-500 ng/mL for rhynchophylline in plasma. The lower limit of quantification for rhynchophylline was 5 ng/mL. The mean recovery of rhynchophylline from plasma was in the range of 87.7-92.6%. The coefficients of variation of intra-day and inter-day precision were both less than 11%. This method is sensitive and selective enough to be used in pharmacokinetic research for the determination of rhynchophylline in rat plasma.
资助项目	Youth Talent Program Foundation of First Affiliated Hospital of Wenzhou Medical College [qnyc010]; key academic subject (clinical Chinese pharmacy) of Twelfth Five Year Program of State Administration of Traditional Chinese Medicine
出版者	OXFORD UNIV PRESS INC
出版地	CARY
ISSN	0021-9665
EISSN	1945-239X
卷号	52 期号:7 页码:661-665
DOI	10.1093/chromsci/bmt096
页数	5
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000359458500002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23839805
SCOPUSEID	2-s2.0-84901460580
通讯作者地址	[Wang, Xianqin]Wenzhou Med Coll, Sch Pharm, Wenzhou 325035, Peoples R China.
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14236
专题	第二临床医学院、附属第二医院、育英儿童医院药学院（分析测试中心）附属第二医院附属第一医院基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Wang, Xianqin
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 2, Wenzhou 325000, Peoples R China; 2.Wenzhou Med Coll, Affiliated Hosp 1, Wenzhou 325000, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China; 4.Wenzhou Med Coll, Sch Pharm, Wenzhou 325035, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Cai, Jinzhang,Lin, Chongliang,Ma, Jianshe,et al. Determination of Rhynchophylline in Rat Plasma by Liquid Chromatography Mass Spectrometry and Its Application[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(7):661-665.
APA	Cai, Jinzhang, Lin, Chongliang, Ma, Jianshe, Hu, Lufeng, Lin, Guanyang, & Wang, Xianqin. (2014). Determination of Rhynchophylline in Rat Plasma by Liquid Chromatography Mass Spectrometry and Its Application. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 52(7), 661-665.
MLA	Cai, Jinzhang,et al."Determination of Rhynchophylline in Rat Plasma by Liquid Chromatography Mass Spectrometry and Its Application".JOURNAL OF CHROMATOGRAPHIC SCIENCE 52.7(2014):661-665.