Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients

doi:10.1016/j.jviromet.2014.09.018

科研成果详情

题名	Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients
作者	Wu, Cun-zao1; Chen, Xiao-qian1; Wang, Zhang-yang 2; Pan, Xiao-dong2; Bai, Yong-heng1; Yang, Yi-rong1; Zheng, Shao-ling1; Xia, Peng1
发表日期	2014-12-15
发表期刊	JOURNAL OF VIROLOGICAL METHODS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cytomegalovirus Polyomavirus Q-PCR Renal transplantation
其他关键词	REAL-TIME PCR ; CYTOMEGALOVIRUS DISEASE ; POLYOMAVIRUS INFECTION ; KIDNEY-TRANSPLANTATION ; VIRUS ; NEPHROPATHY ; REPLICATION ; VIREMIA ; SYSTEM ; BLOOD
摘要	Polyomavirus (BKV) and cytomegalovirus (CMV) are associated with renal graft failure. The aim was to establish a quantitative PCR method (Q-PCR) to detect BIN and CMV simultaneously. The conserved sequences of BIN and CMV were amplified and cloned into the plasmids as standards. The sensitivity, specificity and the precision of the assay were evaluated. Q-PCR was used to detect BIN and CMV DNA simultaneously in 480 blood samples of renal transplantation recipients. The sensitivity of the Q-PCR assay to detect BKV or CMV DNA reached 5 x 10(3) copies/mL. The use of control DNA verified that the assay could specifically detect the target DNA. The precision of the assay to quantify target DNA copies was acceptable (ICV 3.44% for BKV and 2.23% for CMV; differences between batches ICV 4.98% for BKV and 3.76% for CMV). In 480 samples, 130 samples (27.08%) were CMV DNA positive, which was significantly higher than the 64 BICV DNA positive samples (13.33%, p<0.05). BKV or CMV DNA positivity was significantly associated with high concentrations of Tacrolimus (TAC) (p value <0.05). The Q-PCR assay to detect both CMV and BIN DNA simultaneously was developed successfully with high sensitivity, precision, and time-effectiveness for clinical measurement. (C) 2014 Elsevier B.V. All rights reserved.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY12H10004]; Technology Foundation of Zhejiang Province [2013C37046]; Zhejiang Provincial Undergraduate Scientific and Technological Innovation Project and the Fresh Talent Programme [2014R413014]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	0166-0934
EISSN	1879-0984
卷号	210 页码:40-44
DOI	10.1016/j.jviromet.2014.09.018
页数	5
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
WOS记录号	WOS:000345482700008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25261811
SCOPUSEID	2-s2.0-84908542616
通讯作者地址	[Xia, Peng]Transplantation Center, The First Affiliated Hospital of Wenzhou Medical University,2# Fuxue Lane,Wenzhou,325000,China
Scopus学科分类	Virology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14232
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_外科学_外科实验室
通讯作者	Xia, Peng
作者单位	1.Transplantation Center, The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Wenzhou Key Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Wu, Cun-zao,Chen, Xiao-qian,Wang, Zhang-yang,et al. Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients[J]. JOURNAL OF VIROLOGICAL METHODS,2014,210:40-44.
APA	Wu, Cun-zao., Chen, Xiao-qian., Wang, Zhang-yang., Pan, Xiao-dong., Bai, Yong-heng., ... & Xia, Peng. (2014). Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients. JOURNAL OF VIROLOGICAL METHODS, 210, 40-44.
MLA	Wu, Cun-zao,et al."Simultaneous monitoring of CMV and BKV by quantitative PCR in renal transplant recipients".JOURNAL OF VIROLOGICAL METHODS 210(2014):40-44.