Pharmacokinetic Study of Tramadol in Rat by Liquid Chromatography Mass Spectrometry

科研成果详情

题名	Pharmacokinetic Study of Tramadol in Rat by Liquid Chromatography Mass Spectrometry
作者	Lin, Kezhi 1; Liu, Zezheng 2; Yang, Suping 2; Lin, Yingying 2; Wen, Congcong 2; Wang, Zhiyi3; Chen, Chan4
发表日期	2015-12-31
发表期刊	LATIN AMERICAN JOURNAL OF PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	bioavailability LC-MS pharmacokinetics rat plasma tramadol
其他关键词	O-DESMETHYLTRAMADOL ; XENOPUS OOCYTES ; ENANTIOMERIC DETERMINATION ; HUMAN PLASMA ; DOUBLE-BLIND ; METABOLITE ; RELEASE ; PLACEBO ; BRAIN ; PAIN
摘要	Tramadol is a synthetic opioid analgesic with multiple pharmacological actions, and has been widely used for acute, chronic, and neuropathic pain. In this study, a sensitive and selective liquid chromatography mass spectrometry method for the determination of tramadol in rat plasma has been developed. After addition of diazepam as internal standard (IS) and protein precipitation by acetonitrilemethanol (90/10, v/v), chromatographic separation was achieved on a C18 (2.1 mm x 50 mm, 5 mu m) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used for quantification. Calibration plots were linear over the range of 5-2000 ng/mL for tramadol in rat plasma. Mean recoveries of tramadol in rat plasma were in the range of 88.7-94.6%. RSD of intra-day and inter-day precision were both less than 11%. The accuracy of the method ranged from 95.6 to 112.3%. The method was successfully applied to pharmacokinetic study of tramadol after intravenous (5, 10 mg/kg) and oral administration (10, 200 mg/kg) in rats. The bioavailability of tramadol was 59.8% in rats.
出版者	COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES
出版地	LA PLATA
ISSN	0326-2383
卷号	34 期号:5 页码:1016-1021
页数	6
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000358529200022
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84929613796
通讯作者地址	[Chen, Chan]The First Affiliated Hospital, Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Pharmaceutical Science;Drug Discovery
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14228
专题	基础医学院（机能实验教学中心）_基础医学实验教学中心附属第二医院附属第一医院实验动物中心
通讯作者	Chen, Chan
作者单位	1.Medical Experimental Teaching Center, Wenzhou Medical University,Wenzhou,325035,China; 2.Laboratory Animal Centre, Wenzhou Medical University,Wenzhou,325035,China; 3.The Second Affiliated Hospital, Yuying Children’s Hospital, Wenzhou Medical University,Wenzhou,325000,China; 4.The First Affiliated Hospital, Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	基础医学院（机能实验教学中心）_基础医学实验教学中心
通讯作者单位	附属第一医院
第一作者的第一单位	基础医学院（机能实验教学中心）_基础医学实验教学中心
推荐引用方式 GB/T 7714	Lin, Kezhi,Liu, Zezheng,Yang, Suping,et al. Pharmacokinetic Study of Tramadol in Rat by Liquid Chromatography Mass Spectrometry[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2015,34(5):1016-1021.
APA	Lin, Kezhi., Liu, Zezheng., Yang, Suping., Lin, Yingying., Wen, Congcong., ... & Chen, Chan. (2015). Pharmacokinetic Study of Tramadol in Rat by Liquid Chromatography Mass Spectrometry. LATIN AMERICAN JOURNAL OF PHARMACY, 34(5), 1016-1021.
MLA	Lin, Kezhi,et al."Pharmacokinetic Study of Tramadol in Rat by Liquid Chromatography Mass Spectrometry".LATIN AMERICAN JOURNAL OF PHARMACY 34.5(2015):1016-1021.