Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish

doi:10.1016/j.cryobiol.2015.08.016

科研成果详情

题名	Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish
作者	Wang, Gongfa 1; Kang, Ning 1; Gong, Hongmei 1; Luo, Yan 1; Bai, Chenglian1; Chen, Yuanhong1; Ji, Xiaoping1; Huang, Changjiang 1; Dong, Qiaoxiang1,2
发表日期	2015-12
发表期刊	CRYOBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Sperm cryopreservation Zebrafish Cold exposure Uncoupling protein (Ucp2)
其他关键词	OXYGEN SPECIES ROS ; HYDROSTATIC-PRESSURE ; HUMAN SPERMATOZOA ; BOAR SPERMATOZOA ; SEMEN QUALITY ; IN-VITRO ; CRYOPRESERVATION ; ANTIOXIDANTS ; EXPRESSION ; OPTIMIZATION
摘要	Oxidative stress plays an important role in sperm damage during cryopreservation. Mild mitochondrial uncoupling has been shown to reduce excessive reactive oxygen species (ROS) and thus mitigate oxidative stress. Uncoupling protein (Ucp2) regulates mitochondrial uncoupling and can be induced by temperature fluctuation. In the present study, we explored a novel approach of acute cold exposure on Ucp2 activation and its association with oxidative damage and post-thaw sperm quality in zebrafish. Our study revealed that acute cold exposure of zebrafish at 18 degrees C for 24 h led to significant increase of ucp2 mRNA and Ucp2 protein in zebrafish fresh sperm as well as thawed sperm after cryopreservation. Although cold exposure had no effect on fresh sperm quality except for decreasing lipid peroxidation, sperm collected from cold-exposed zebrafish exhibited higher resistance to cryodamage, which was demonstrated by increased post-thaw motility, decreased lipid peroxidation, increased ATP production, and ultimately increased fertilization success. However, except for reduced lipid peroxidation, we did not observe any significant ROS reduction associated with increased Ucp2 activation in cold-exposed group, suggesting mechanisms other than mitochondrial uncoupling could have contributed to cold exposure associated benefits in post-thaw sperm survival. Nevertheless, our findings indicate that acute cold exposure prior to sperm cryopreservation is beneficial for post-thaw sperm survival in zebrafish, and this novel approach may be used to improve post-thaw sperm quality for other aquatic species. (C) 2015 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31172386]
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0011-2240
EISSN	1090-2392
卷号	71 期号:3 页码:464-471
DOI	10.1016/j.cryobiol.2015.08.016
页数	8
WOS类目	Biology ; Physiology
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Physiology
WOS记录号	WOS:000367496800013
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26408847
SCOPUSEID	2-s2.0-84959088260
通讯作者地址	[Dong, Qiaoxiang]Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Agricultural and Biological Sciences (all)
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14214
专题	公共卫生学院_环境安全与健康风险评估研究院检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Dong, Qiaoxiang
作者单位	1.Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China; 2.School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	公共卫生学院_环境安全与健康风险评估研究院
通讯作者单位	公共卫生学院_环境安全与健康风险评估研究院
第一作者的第一单位	公共卫生学院_环境安全与健康风险评估研究院
推荐引用方式 GB/T 7714	Wang, Gongfa,Kang, Ning,Gong, Hongmei,et al. Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish[J]. CRYOBIOLOGY,2015,71(3):464-471.
APA	Wang, Gongfa., Kang, Ning., Gong, Hongmei., Luo, Yan., Bai, Chenglian., ... & Dong, Qiaoxiang. (2015). Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish. CRYOBIOLOGY, 71(3), 464-471.
MLA	Wang, Gongfa,et al."Upregulation of uncoupling protein Ucp2 through acute cold exposure increases post-thaw sperm quality in zebrafish".CRYOBIOLOGY 71.3(2015):464-471.