哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义

doi:10.3760/cma.j.issn.1008-6706.2015.23.012

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题名	哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义
其他题名	Roles of GITR/GITRL signal system expression at bloodstream lymphocytes in children with asthma
作者	方丽 1,2; 李蓉蓉 1,2; 骆方军1,2; 赵丽燕 1,2; 陈培英 1,2; 俞培锋 1,2
发表日期	2015
发表期刊	中国基层医药影响因子和分区
语种	中文
原始文献类型	Periodical
关键词	哮喘 T 淋巴细胞受体肿瘤坏死因子信号传导
其他关键词	Asthma ; T lymphocytes ; Receptor ; tumor necrosis factor ; Signal transduction
摘要	目的：观察 GITR 及其配体（GITRL）在哮喘患儿外周血淋巴细胞的表达，探讨哮喘的部分炎症机制。方法50例儿童重度哮喘患儿（哮喘组）分别在急性期和缓解期取静脉血，流式细胞术检测CD ＋4 CD25＋Treg 细胞 GITR 和 CD ＋4 T 细胞 GITRL 的平均荧光强度，与32例健康儿童作对照，进行统计分析。结果哮喘组急性期 CD ＋4 CD25＋Treg 细胞 GITR 的表达水平为（24．2±8．2）MFI，显著低于对照组的（28．5±6．0）MFI（t ＝2．5，P ＜0．05）。哮喘组急性期与对照组 CD ＋4 T 细胞 GITRL 的表达水平分别为（5．2±3．6）MFI与（4．6±1．0）MFI，差异无统计学意义（t ＝1．1，P ＞0．05）。哮喘组 CD ＋4 CD25＋Treg 细胞 GITR 的表达水平在缓解期[（29．5±8．3）MFI]显著高于急性期（t ＝－9．9，P ＜0．01）；CD ＋4 T 细胞 GITRL 的表达水平在哮喘组急性期、缓解期（5．7±3．6）MFI 之间差异无统计学意义（t ＝－1．6，P ＞0．05）。CD ＋4 CD25＋Treg GITR 与 CD ＋4 T GITRL 的表达之间无显著相关性（n ＝132，r ＝0．04，P ＞0．05）。结论哮喘急性期患儿 CD ＋4 CD25＋Treg 细胞GITR 表达下降，在缓解期得到回升，而 CD ＋4 T 细胞 GITRL 的表达水平没有变化，GITR／GITRL 信号系统可能参与了哮喘的炎症过程。
其他摘要	Objective To investigate the expression of blood CD +4 CD +25 Treg GITR,CD +4 T cell GITRL in children with asthma,and the role of them in asthmatic inflammation.Methods 50 cases of severe asthma were selected,and were controlled with thirty two healthy children.The venous blood was collected both in the period of acute episode and clinic remission.The mean fluorescence intensity of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL was detected by flow cytometry.Results The expression of CD +4 CD +25 Treg GITR in the asthma acute period group was (24.2 ±8.2)MFI,which was significantly lower than (28.5 ±6.0)MFI in the control group(t =2.5,P <0.05).While there was no significant difference of CD +4 T cell GITRL expression between the asthma acute period group[(5.2 ±3.6)MFI]and the control group[(4.6 ±1.0)MFI](t =1.1,P 0.05).Moreover,the expression of CD +4 CD +25 Treg GITR in the asthma in remission group after treatment was (29.5 ±8.3)MFI,which was significantly higher than that in acute period group before treatment(t =-9.9,P <0.01 ).Whereas,there was no significant difference of CD +4 T cell GITRL expression in asthma group between in remission after treatment[(5.7 ±3.6)MFI] and in acute period before treatment(t =-1.6,P 0.05).Furthermore,there was no significant correlation between levels of CD +4 CD +25 Treg GITR and CD +4 T cell GITRL.Conclusion The level of CD +4 CD +25 Treg GITR in acute period asthmatic patients was decreased,but it was increased in remission,but no changes of CD +4 T cell GITRL expression were observed.GITR/GITRL signal system might be involved in the asthmatic inflammation procession.
资助项目	浙江省诸暨市科技局基金资助项目;
ISSN	1008-6706
卷号	22 期号:23 页码:3558-3560
DOI	10.3760/cma.j.issn.1008-6706.2015.23.012
页数	3
收录类别	万方 ; 维普 ; ISTIC
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/141338
专题	其他_附属诸暨医院（诸暨市人民医院）
作者单位	1.温州医科大学附属诸暨医院儿科, 浙江省诸暨,311800; 2.温州医科大学附属诸暨医院检验科, 浙江省诸暨,311800
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	方丽,李蓉蓉,骆方军,等. 哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义[J]. 中国基层医药,2015,22(23):3558-3560.
APA	方丽, 李蓉蓉, 骆方军, 赵丽燕, 陈培英, & 俞培锋. (2015). 哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义. 中国基层医药, 22(23), 3558-3560.
MLA	方丽,et al."哮喘患儿血淋巴细胞糖皮质激素诱导的肿瘤坏死因子受体及其配体信号系统的表达及意义".中国基层医药 22.23(2015):3558-3560.