斑秃患者外周血CD4+CD25+T细胞培养上清液白介素10和转化生长因子β1检测

doi:10.3760/cma.j.issn.0412-4030.2014.01.017

科研成果详情

题名	斑秃患者外周血CD4+CD25+T细胞培养上清液白介素10和转化生长因子β1检测
其他题名	Detection of interleukin-10 and transforming growth factor-β1 in the culture supernatant of CD4+CD25+ T cells from patients with alopecia areata
作者	马新华; 邵文俊; 金宛宛; 高宇
发表日期	2014-01-30
发表期刊	中华皮肤科杂志影响因子和分区
语种	中文
原始文献类型	Periodical ; 期刊论文
关键词	斑秃转化生长因子β1 白细胞介素10
其他关键词	Alopecia areata ; Transforming growth factor beta1 ; Interleukin-10
摘要	目的探讨CD4+CD25+T细胞与斑秃发病之间的关系.方法收集了3组研究对象,其中健康对照组25例、稳定期斑秃患者25例、进展期斑秃患者23例.抽取所有对象外周血,提取CD4+CD25+T细胞,培养4d,收集培养上清液,ELISA法检测上清液IL-10和TGF-β1水平.结果进展期斑秃患者外周血CD4+CD25+T细胞培养的IL-10和TGF-β1分别为(31.68±6.78) pg/ml和(32.29±6.80) pg/ml,明显低于健康对照组(57.34±14.15) pg/ml、(57.43±15.16) pg/ml和稳定期斑秃患者(52.56±13.02) pg/ml和(61.75±14.10) pg/ml(P＜ 0.05),而健康对照组和稳定期斑秃患者之间差异无统计学意义(P＞0.05).结论 CD4+CD25+T细胞分泌IL-10和TGF-β1功能降低,在斑秃发病中有一定意义.
其他摘要	Objective To evaluate the potential association of CD4+CD25+ T cells with alopecia areata.Methods Totally,this study enrolled 23 patients with progressive alopecia areata,25 patients with stable alopecia areata,and 25 healthy controls.Peripheral blood was isolated from these subjects followed by isolation of CD4+ CD25+ regulatory T cells,which were then cuhured with the presence of anti-CD3 and-CD28 monoclonal antibodies for four days.Subsequently,enzyme-linked immunosorbent assay was performed to measure the levels of interleukin (IL)-10 and transforming growth factor (TGF)-β1 in the culture supematant of these T cells.Results The levels of IL-10 and TGF-β1 were (31.68 ± 6.78) pg/ml and (32.29 ± 6.8) pg/ml respectively in the culture supernatant of CD4+CD25+ regulatory T cells from patients with progressive alopecia areata,significantly lower than those from the healthy controls ((57.34 ± 14.15) pg/ml and (57.43 ± 15.16) pg/ml,both P ＜ 0.05) and patients with stable alopecia areata ((52.56 ± 13.02) pg/ml and (61.75 ± 14.10) pg/ml,both P ＜ 0.05).However,no significant difference was observed in the supernatant levels of IL-10 or TGF-β1 between the healthy controls and patients with stable alopecia areata.Conclusions The secretion of IL-10 and TGF-β1 by CD4+CD25+ T cells is decreased in patients with progressive alopecia areata,which may contribute to the pathogenesis of alopecia areata.
资助项目	浙江省温州市科技局对外合作课题;
ISSN	0412-4030
卷号	47 期号:1 页码:55-56
DOI	10.3760/cma.j.issn.0412-4030.2014.01.017
页数	2
收录类别	万方 ; 维普 ; ISTIC ; PKU ; 北大核心 ; CSCD
学科领域	医药、卫生 ; 皮肤病学与性病学 ; 特种医学
URL	查看原文
CSCD记录号	CSCD:5041832
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/140841
专题	附属第二医院_皮肤科
作者单位	325027,温州医科大学附属第二医院皮肤科
第一作者单位	附属第二医院_皮肤科
第一作者的第一单位	附属第二医院_皮肤科
推荐引用方式 GB/T 7714	马新华,邵文俊,金宛宛,等. 斑秃患者外周血CD4+CD25+T细胞培养上清液白介素10和转化生长因子β1检测[J]. 中华皮肤科杂志,2014,47(1):55-56.
APA	马新华, 邵文俊, 金宛宛, & 高宇. (2014). 斑秃患者外周血CD4+CD25+T细胞培养上清液白介素10和转化生长因子β1检测. 中华皮肤科杂志, 47(1), 55-56.
MLA	马新华,et al."斑秃患者外周血CD4+CD25+T细胞培养上清液白介素10和转化生长因子β1检测".中华皮肤科杂志 47.1(2014):55-56.