MicroRNA-1275 induces radiosensitization in oesophageal cancer by regulating epithelial-to-mesenchymal transition via Wnt/beta-catenin pathway

doi:10.1111/jcmm.14784

科研成果详情

题名	MicroRNA-1275 induces radiosensitization in oesophageal cancer by regulating epithelial-to-mesenchymal transition via Wnt/beta-catenin pathway
作者	Xie, Congying1; Wu, Youyi 2; Fei, Zhenghua1; Fang, Ya1; Xiao, Shenlan 1; Su, Huafang1
发表日期	2020-01
发表期刊	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	epithelial-mesenchymal transition miR-1275 oesophageal cancer radioresistance Wnt beta-catenin
其他关键词	SQUAMOUS-CELL CARCINOMA ; DNA-DAMAGE ; RADIORESISTANCE ; GROWTH ; PROLIFERATION ; INVASION ; ZEB1 ; EMT ; EXPRESSION ; REPRESSION
摘要	Acquired radioresistance is one of the main obstacles for the anti-tumour efficacy of radiotherapy in oesophageal cancer (EC). Recent studies have proposed microRNAs (miRNAs) as important participators in the development of radioresistance in various cancers. Here, we investigated the role of miR-1275 in acquired radioresistance and epithelial-mesenchymal transition (EMT) in EC. Firstly, a radioresistant cell line KYSE-150R was established, with an interesting discovery was observed that miR-1275 was down-regulated in KYSE-150R cells compared to the parental cells. Functionally, miR-1275 inhibition elevated radioresistance in KYSE-150 cells via promoting EMT, whereas enforced expression of miR-1275 increased radiosensitivity in KYSE-150R cells by inhibiting EMT. Mechanically, we demonstrated that miR-1275 directly targeted WNT1 and therefore inactivated Wnt/beta-catenin signalling pathway in EC cells. Furthermore, WNT1 depletion countervailed the promoting effect of miR-1275 suppression on KYSE-150 cell radioresistance through hampering EMT, whereas WNT1 overexpression rescued miR-1275 up-regulation-impaired EMT to reduce the sensitivity of KYSE-150R cells to radiation. Collectively, our findings suggested that miR-1275 suppressed EMT to encourage radiosensitivity in EC cells via targeting WNT1-activated Wnt/beta-catenin signalling, providing a new therapeutic outlet for overcoming radioresistance of patients with EC.
资助项目	Natural Science Foundation of China[81602658];Natural Science Foundation of Zhejiang Province[LQ15H160013,LY17H160051];
出版者	WILEY
出版地	HOBOKEN
ISSN	1582-1838
EISSN	1582-4934
卷号	24 期号:1 页码:747-759
DOI	10.1111/jcmm.14784
页数	13
WOS类目	Cell Biology ; Medicine, Research & Experimental
WOS研究方向	Cell Biology ; Research & Experimental Medicine
WOS记录号	WOS:000505309400063
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	31733028
PMC记录号	PMC6933350
SCOPUSEID	2-s2.0-85075143350
通讯作者地址	[Su, Huafang]Department of Radiotherapy and Chemotherapy,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Molecular Medicine;Cell Biology
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13966
专题	附属第一医院其他_附属第三医院（瑞安市人民医院）
通讯作者	Su, Huafang
作者单位	1.Department of Radiotherapy and Chemotherapy,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Departments of Radiation Oncology,The Third Affiliated Hospital of Wenzhou Medical University,Ruian,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Xie, Congying,Wu, Youyi,Fei, Zhenghua,et al. MicroRNA-1275 induces radiosensitization in oesophageal cancer by regulating epithelial-to-mesenchymal transition via Wnt/beta-catenin pathway[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020,24(1):747-759.
APA	Xie, Congying, Wu, Youyi, Fei, Zhenghua, Fang, Ya, Xiao, Shenlan, & Su, Huafang. (2020). MicroRNA-1275 induces radiosensitization in oesophageal cancer by regulating epithelial-to-mesenchymal transition via Wnt/beta-catenin pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 24(1), 747-759.
MLA	Xie, Congying,et al."MicroRNA-1275 induces radiosensitization in oesophageal cancer by regulating epithelial-to-mesenchymal transition via Wnt/beta-catenin pathway".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 24.1(2020):747-759.