先天性心脏病伴肺动脉高压肺组织中腺泡内动脉转化生长因子-β1 mRNA和内皮素-1 mRNA的表达及意义

doi:10.3760/j.issn:0529-5807.2005.03.008

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题名	先天性心脏病伴肺动脉高压肺组织中腺泡内动脉转化生长因子-β1 mRNA和内皮素-1 mRNA的表达及意义
其他题名	Expression and pathological implication of transforming growth factor- β1 mRNA and endothelin-1 mRNA in intraacinar pulmonary arterioles of congenital heart disease accompanied with pulmonary hypertension
作者	高宝辉1,2,3; 姚荣欣1,2,3; 赵志光1,2,3; 谢丽微1,2,3; 项如莲1,2,3; 胡型锑1,2,3; 王宗敏1,2,3
发表日期	2005
发表期刊	中华病理学杂志影响因子和分区
语种	中文
原始文献类型	Periodical ; 学术期刊 ; 期刊论文
关键词	转化生长因子β 内皮缩血管肽-1 高血压肺性心脏缺损先天性高血压,肺性心脏缺损,先天性
摘要	目的探讨转化生长因子-β1(TGF-β1)和内皮素-1(ET-1)在先天性心脏病(先心病)伴肺动脉高压(PH)的肺组织腺泡内肺动脉中的表达及意义.方法收集51例肺组织,其中41例为左向右分流型先心病患儿[伴PH 25例(A组),无PH 16例(B组)],对照组10例(C组),应用原位杂交、图像分析检测51例腺泡内肺动脉(IAPA)TGF-β1 mRNA和ET-1 mRNA的表达及平均吸光度值(A值);透射电镜观察肺组织超微结构;弹力纤维(VG)染色显示腺泡内肺动脉并计算其数量的变化.结果 (1)肺腺泡内部分肌型及环肌型动脉的数量,A、B两组分别与C组比较, F值分别为149.96、142.01,P<0.01;(2)电镜观察,有的肺小动脉内皮细胞增生;中膜平滑肌增厚,平滑肌细胞面积增大;外膜胶原纤维密集;毛细血管基膜增厚;(3)原位杂交发现,TGF-β1 mRNA在A、B两组腺泡内肺动脉均有阳性表达,A值分别为(0.1988±0.0498)、(0.1098±0.0428),C组表达微弱(A值=0.0578±0.0096),A、B、C三组间比较差异有统计学意义(P<0.05);ET-1 mRNA 在A、B两组肺腺泡内肺动脉内皮细胞表达明显增强,A值分别为(0.1692±0.0205)、(0.1004±0.0140),C组仅有微弱表达(A值=0.0746±0.0119),A、B、C三组间比较差异有统计学意义(P<0.01).结论左向右分流型先心病患儿肺组织腺泡内部分肌型及肌型动脉数量明显增加,肺血管结构重组,TGF-β1 mRNA、ET-1 mRNA表达增高与先心病伴PH的发生有关系.
其他摘要	Objective To study the expression and pathological implication of transforming growth factor-1 (TGF-1) and endothelin-1 ( ET-1) in intraacinar pulmonary arterioles of children with congenital heart disease and pulmonary hypertension (HP). Methods Forty-one children with left-to-right shunt congenital heart disease were studied including 25 cases of HP (group A) , 16 cases without HP (group B) and 10 children without congenital heart disease as the contols (group C). Expression of TGF-βl mRNA and ET-1 mRNA in intraacinar pulmonary arteriolar (IAPA) was studied using in-situ hybridization and image pattern analysis of their absorption values (A value). Changes of the intraacinar arterioles and lung tissue were studied by elastic fiber staining and electronic microscopy respectively. Results (1) There was a significant difference in the amount of intraacinar pulmonary arterioles ( partial-muscular and muscular) counted in either group A or B in comparing with that of group C (F values 149. 96 and 142. 01 respectively, P < 0. 01 ) ; (2) Electronic microscopy demonstrated endothelial proliferation of the small arteries, thickening of arteriolar wall, increased density of collagen fibers at adventitia and increased thickness of the capillary basal membrane; (3) The A value of TGF-pl mRNA expressed in the pulmonary arterioles of groups A and B by in-situ hybridization were 0. 1988 ±0, 0498 and 0. 1098 ±0. 0428 respectively, however, the expression was weak in group C (A value; 0. 0578 ±0. 0096). There were all significant between each two groups (F=45. 95, P <0. 01). The expression of ET-1 mRNA was markedly increased as well in the endothelial cells of pulmonary arterioles in both groups A and B, with A values of 0. 1692 ± 0. 0205 and 0. 1004 ±0. 0140 respectively, whereas the expression was weak in group C (A value of 0. 0746 ±0. 0119). There were all significant between each two groups (F = 139. 996, P < 0. 01). Conclusions The number of intraacinar pulmonary partial-muscular and muscular arterioles in patients with left-to-right shunt congenital heart defect is drastically increased, along with marked restructuring of the pulmonary vasculatures. In addition, there seems a correlation present between the overexpression of TGF-βl mRNA and ET-1 mRNA in intraacinar pulmonary arterioles and the occun-ence of pulmonary hypertenaion in patients with congenital heart disease.
资助项目	浙江省温州市科技局资助项目;
ISSN	0529-5807
卷号	34 期号:3 页码:159-162
DOI	10.3760/j.issn:0529-5807.2005.03.008
页数	4
收录类别	万方 ; PKU ; ISTIC ; CNKI ; 北大核心 ; CSCD
学科领域	医药、卫生 ; 基础医学
URL	查看原文
CSCD记录号	CSCD:1958736
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/138451
专题	第二临床医学院，附属第二医院、育英儿童医院_病理学_病理科第二临床医学院，附属第二医院、育英儿童医院
作者单位	1.325027,温州,浙江温州医学院附属育英儿童医院病理科; 2.325027,温州,浙江温州医学院附属育英儿童医院心内科; 3.325027,温州,浙江温州医学院附属育英儿童医院胸外科
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院_病理学_病理科; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院_病理学_病理科
推荐引用方式 GB/T 7714	高宝辉,姚荣欣,赵志光,等. 先天性心脏病伴肺动脉高压肺组织中腺泡内动脉转化生长因子-β1 mRNA和内皮素-1 mRNA的表达及意义[J]. 中华病理学杂志,2005,34(3):159-162.
APA	高宝辉., 姚荣欣., 赵志光., 谢丽微., 项如莲., ... & 王宗敏. (2005). 先天性心脏病伴肺动脉高压肺组织中腺泡内动脉转化生长因子-β1 mRNA和内皮素-1 mRNA的表达及意义. 中华病理学杂志, 34(3), 159-162.
MLA	高宝辉,et al."先天性心脏病伴肺动脉高压肺组织中腺泡内动脉转化生长因子-β1 mRNA和内皮素-1 mRNA的表达及意义".中华病理学杂志 34.3(2005):159-162.

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