microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2

doi:10.1016/j.gene.2017.07.046

科研成果详情

题名	microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2
作者	Huang, Chongqing; Huang, JingYong; Ma, Pengyan; Yu, Guanfeng
发表日期	2017-09-10
发表期刊	GENE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Apoptosis Bcl-2 Hemangioma miR-143 Proliferation
其他关键词	CELL LUNG-CANCER ; DOWN-REGULATION ; INFANTILE HEMANGIOMA ; ENDOTHELIAL-CELLS ; PROLIFERATION ; APOPTOSIS ; PATHWAY ; EXPRESSION ; MIR-143 ; PROPRANOLOL
摘要	Infantile hemangioma is the most common vascular tumor affecting infants, which is associated with clonal expansion of endothelial cells. The aim of this study is to determine the role of microRNA (miR)-143 in the growth and survival of hemangioma-derived endothelial cells (HemECs). We examined the expression of miR-143 in patients with proliferating-phase (n = 10) and involuting-phase (n = 8) hemangiomas. The effects of ectopic expression of miR-143 on the viability, proliferation, cell cycle distribution, and apoptosis of HemECs were explored. We also identified the target gene(s) that was involved in the activity of miR-143. It was found that proliferating hemangiomas had significantly (P < 0.05) lower levels of miR-143 than involuting counterparts. Reexpression of miR-143 significantly reduced the viability and proliferation of HemECs, while knockdown of miR-143 led to an increase in the proliferation of HemECs. Moreover, overexpression of miR-143 arrested HemECs at the G0/G1 phase and promoted caspase-3-dependent apoptosis. At the molecular level, miR-143 overexpression significantly promoted the expression of p21 and p53 and reduced the expression of cyclin D1, CDK2, CDK4, and Bcl-2. Silencing of Bcl-2 phenocopied the effect of miR-143 overexpression on the proliferation and apoptosis of HemECs. Furthermore, co-expression of Bcl-2 reversed the growth-suppressive effect of miR-143 on HemECs. Taken together, miR-143 acts as a suppressor in the growth of HemECs, at least partially, through downregulation of Bcl-2. Reexpression of miR-143 may represent a potential therapeutic strategy for the treatment of proliferating hemangiomas.
资助项目	Wenzhou City Public Science and Technology Project of China [Y20160407]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	0378-1119
EISSN	1879-0038
卷号	628 页码:211-217
DOI	10.1016/j.gene.2017.07.046
页数	7
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000412256800029
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28716710
SCOPUSEID	2-s2.0-85024884354
通讯作者地址	[Huang, Chongqing]Department of Vascular Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Genetics
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13835
专题	附属第一医院_血管外科
通讯作者	Huang, Chongqing
作者单位	Department of Vascular Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, Chongqing,Huang, JingYong,Ma, Pengyan,et al. microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2[J]. GENE,2017,628:211-217.
APA	Huang, Chongqing, Huang, JingYong, Ma, Pengyan, & Yu, Guanfeng. (2017). microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2. GENE, 628, 211-217.
MLA	Huang, Chongqing,et al."microRNA-143 acts as a suppressor of hemangioma growth by targeting Bcl-2".GENE 628(2017):211-217.