Shikonin regulates autophagy via the AMPK/mTOR pathway and reduces apoptosis of human umbilical cord mesenchymal stem cells to improve survival in tissues surrounding brain contusion

doi:10.3892/etm.2021.10910

科研成果详情

题名	Shikonin regulates autophagy via the AMPK/mTOR pathway and reduces apoptosis of human umbilical cord mesenchymal stem cells to improve survival in tissues surrounding brain contusion
作者	Zhu, Xiaohong1,2; Huang, Lijie1,2; Wu, Ke 1,2; Sun, Zhezhe 1,2; Wang, Kankai1,2; Ru, Junnan 1,2; Zhuge, Qichuan1,2; Ruan, Linhui1,2
发表日期	2021-12
发表期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	shikonin human umbilical cord mesenchymal stem cells autophagy apoptosis traumatic brain injury
其他关键词	NF-KAPPA-B ; ISCHEMIA/REPERFUSION INJURY ; ACTIVATION ; MECHANISMS ; STRESS ; DEATH ; NUTRIENT ; TARGET ; AMPK
摘要	Shikonin has been reported to regulate autophagy via the AMP-activated protein kinase (AMPK)/mTOR signalling pathway and decrease apoptosis in transplanted human umbilical cord mesenchymal stem cells (HUMSCs). In the present study, HUMSCs were exposed to oxygen glucose deprivation (OGD) in vitro for 12 h, and TUNEL fluorescence staining was used to detect apoptosis. Differences in autophagy and AMPK/mTOR pathway-related protein expression following treatment with shikonin were quantitatively analyzed by western blotting. Green fluorescent protein-labelled stem cells were implanted into traumatic brain injury-model mice and the survival of HUMSCs was observed after 7 days. Shikonin increased the number of cells in brain tissue surrounding the contusion 7 days after transplantation. Furthermore, shikonin treatment decreased apoptosis, increased the expression of autophagy-related proteins, increased phosphorylated AMPK expression and downregulated phosphorylated mTOR expression. In addition, the autophagy inhibitor 3-methyladenine attenuated these effects and aggravated apoptosis. Subsequently, shikonin upregulated autophagy and protected HUMSCs in the area surrounding contused brain tissue. Shikonin may regulate autophagy via the AMPK/mTOR signalling pathway and protect transplanted HUMSCs from apoptosis induced by hypoxia/ischemia.
资助项目	Municipal Science and Technology Bureau of Wenzhou [Y20190568]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1792-0981
EISSN	1792-1015
卷号	22 期号:6 页码:1475
DOI	10.3892/etm.2021.10910
页数	9
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000716985500001
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	34765016
PMC记录号	PMC8576632
通讯作者地址	[Zhuge, Qichuan;Ruan, Linhui]Wenzhou Med Univ, Dept Neurosurg, Affiliated Hosp 1, 113 Fanhaixi Rd, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13588
专题	附属第一医院_神经外科第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Zhuge, Qichuan; Ruan, Linhui
作者单位	1.Wenzhou Med Univ, Dept Neurosurg, Affiliated Hosp 1, 113 Fanhaixi Rd, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Zhejiang Prov Key Lab Aging & Neurol Disorder Res, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 神经外科
通讯作者单位	附属第一医院; 神经外科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhu, Xiaohong,Huang, Lijie,Wu, Ke,et al. Shikonin regulates autophagy via the AMPK/mTOR pathway and reduces apoptosis of human umbilical cord mesenchymal stem cells to improve survival in tissues surrounding brain contusion[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2021,22(6):1475.
APA	Zhu, Xiaohong., Huang, Lijie., Wu, Ke., Sun, Zhezhe., Wang, Kankai., ... & Ruan, Linhui. (2021). Shikonin regulates autophagy via the AMPK/mTOR pathway and reduces apoptosis of human umbilical cord mesenchymal stem cells to improve survival in tissues surrounding brain contusion. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 22(6), 1475.
MLA	Zhu, Xiaohong,et al."Shikonin regulates autophagy via the AMPK/mTOR pathway and reduces apoptosis of human umbilical cord mesenchymal stem cells to improve survival in tissues surrounding brain contusion".EXPERIMENTAL AND THERAPEUTIC MEDICINE 22.6(2021):1475.