Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application

doi:10.1002/bmc.1578

科研成果详情

题名	Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application
作者	Lin, Guanyang2; Ma, Jianshe3; Zhu, Jiayin4; Yang, Xuezhi2; Hu, Lufeng2; Wang, Xianqin1
发表日期	2011-10
发表期刊	BIOMEDICAL CHROMATOGRAPHY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	LC-MS/MS azasetron hydrochloride pharmacokinetics plasma
其他关键词	Y-25130
摘要	A sensitive and selective liquid chromatography tandem mass spectrometry method for determination of azasetron hydrochloride in rabbit plasma was developed. After addition of doxapram hydrochloride as internal standard (IS), protein precipitation by 10% trichloroacetic acid was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C(18) (2.1 50 mm, 3.5 mm) column with acetonitrile-water as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used to quantification using target fragment ions m/z 349.9 -> 223.5 for azasetron hydrochloride and m/z 378.9 -> 291.8 for the IS. Calibration plots were linear over the range of 6-1000 ng/mL for azasetron hydrochloride in plasma. The lower limit of quantitation for azasetron hydrochloride was 6 ng/mL. The mean recovery of azasetron hydrochloride from plasma was in the range 85.6-92.7%. The RSDs of intra-day and inter-day precision were both less than 12%. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of azasetron hydrochloride in rabbit plasma. Copyright (C) 2010 John Wiley & Sons, Ltd.
资助项目	Analyzing and Testing Foundation of Zhejiang Province [2007F80010]
出版者	WILEY-BLACKWELL
出版地	MALDEN
ISSN	0269-3879
EISSN	1099-0801
卷号	25 期号:10 页码:1107-1111
DOI	10.1002/bmc.1578
页数	5
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS记录号	WOS:000295143500006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	21204117
SCOPUSEID	2-s2.0-80052522677
通讯作者地址	[Wang, Xianqin]Wenzhou Med Coll, Analyt & Testing Ctr, University Town 325035, Wenzhou, Peoples R China.
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13569
专题	附属第一医院实验动物中心基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Wang, Xianqin
作者单位	1.Wenzhou Med Coll, Analyt & Testing Ctr, University Town 325035, Wenzhou, Peoples R China; 2.Wenzhou Med Coll, Affiliated Hosp 1, Wenzhou 325000, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China; 4.Wenzhou Med Coll, Lab Anim Ctr, Wenzhou 325035, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Guanyang,Ma, Jianshe,Zhu, Jiayin,et al. Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application[J]. BIOMEDICAL CHROMATOGRAPHY,2011,25(10):1107-1111.
APA	Lin, Guanyang, Ma, Jianshe, Zhu, Jiayin, Yang, Xuezhi, Hu, Lufeng, & Wang, Xianqin. (2011). Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application. BIOMEDICAL CHROMATOGRAPHY, 25(10), 1107-1111.
MLA	Lin, Guanyang,et al."Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application".BIOMEDICAL CHROMATOGRAPHY 25.10(2011):1107-1111.