Theaflavin protects chondrocytes against apoptosis and senescence via regulating Nrf2 and ameliorates murine osteoarthritis

doi:10.1039/d0fo02038a

科研成果详情

题名	Theaflavin protects chondrocytes against apoptosis and senescence via regulating Nrf2 and ameliorates murine osteoarthritis
作者	Xu, Xin-Xian1,2; Zheng, Gang1,2; Tang, Shang-Kun 1,2,3; Liu, Hai-Xiao1,2; Hu, Yue-Zheng1,2; Shang, Ping2,4
发表日期	2021-02-21
发表期刊	FOOD & FUNCTION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Metabolism Tea Beneficial effects Degenerative disease Enzyme linked immunosorbent assay Immunofluorescent staining Molecular binding Oral administration Protective effects Tert-butylhydroperoxide
摘要	Oxidative stress-mediated excessive apoptosis and senescence of chondrocytes are the main pathological alterations in the osteoarthritis (OA) development. The protective effects of theaflavin (TF), a common group of polyphenols in black tea, against many degenerative diseases by attenuating oxidative stress are well reported. Nevertheless, its role in the OA treatment is still scantily understood. In the current research, by applying enzyme-linked immunosorbent assay (ELISA) kits and immunofluorescent staining, TF treatment was found to inhibit tert-Butyl hydroperoxide (TBHP)-induced imbalance of anabolism and catabolism in primary mouse chondrocytes. Then, according to western blot, live-dead staining, and SA-beta-gal staining, the dramatically increased level of apoptosis and senescence of chondrocytes in response to TBHP was also found to be reduced by TF administration. With regard to upstream signaling investigation, the in vitro molecular binding analysis indicated that the beneficial effects of TF might be related to the regulation of the Keap1/Nrf2/HO-1 axis. Furthermore, the Silencing of Nrf2 resulted in the abolishment of the anti-apoptosis and anti-senescence effects of TF. In addition, the oral administration of TF was demonstrated to ameliorate osteoarthritis development in a surgically induced mouse OA model. Taken together, these results suggest that TF might be a promising therapeutic option for the treatment of OA.
资助项目	Wenzhou science and technology planning project [Y20190520]; General Project of Education Department of Zhejiang Province [Y202043146]
出版者	ROYAL SOC CHEMISTRY
出版地	CAMBRIDGE
ISSN	2042-6496
EISSN	2042-650X
卷号	12 期号:4 页码:1590-1602
DOI	10.1039/d0fo02038a
页数	13
WOS类目	Biochemistry & Molecular Biology ; Food Science & Technology
WOS研究方向	Biochemistry & Molecular Biology ; Food Science & Technology
WOS记录号	WOS:000623956200016
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20211010028363
EI主题词	Cell death
URL	查看原文
PubMed ID	33471008
SCOPUSEID	2-s2.0-85101899171
通讯作者地址	[Hu, Yue-Zheng]Wenzhou Med Univ, Dept Orthopaed Surg, Affiliated Hosp 2, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China. ; [Hu, Yue-Zheng;Shang, Ping]Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China. ; [Shang, Ping]Wenzhou Med Univ, Dept Rehabil, Affiliated Hosp 2, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China.
Scopus学科分类	Food Science
TOP期刊	TOP期刊
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1351
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院附属第二医院_康复科
通讯作者	Hu, Yue-Zheng; Shang, Ping
作者单位	1.Wenzhou Med Univ, Dept Orthopaed Surg, Affiliated Hosp 2, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China; 3.Wenzhou Med Univ, Sch Med 2, Wenzhou, Peoples R China; 4.Wenzhou Med Univ, Dept Rehabil, Affiliated Hosp 2, 109 Xueyuanxi Rd, Wenzhou 325027, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院; 康复科
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Xu, Xin-Xian,Zheng, Gang,Tang, Shang-Kun,et al. Theaflavin protects chondrocytes against apoptosis and senescence via regulating Nrf2 and ameliorates murine osteoarthritis[J]. FOOD & FUNCTION,2021,12(4):1590-1602.
APA	Xu, Xin-Xian, Zheng, Gang, Tang, Shang-Kun, Liu, Hai-Xiao, Hu, Yue-Zheng, & Shang, Ping. (2021). Theaflavin protects chondrocytes against apoptosis and senescence via regulating Nrf2 and ameliorates murine osteoarthritis. FOOD & FUNCTION, 12(4), 1590-1602.
MLA	Xu, Xin-Xian,et al."Theaflavin protects chondrocytes against apoptosis and senescence via regulating Nrf2 and ameliorates murine osteoarthritis".FOOD & FUNCTION 12.4(2021):1590-1602.