The prevalence and mechanism of triclosan resistance inEscherichia coliisolated from urine samples in Wenzhou, China

doi:10.1186/s13756-020-00823-5

科研成果详情

题名	The prevalence and mechanism of triclosan resistance inEscherichia coliisolated from urine samples in Wenzhou, China
作者	Zeng, Weiliang 1; Xu, Wenya 2; Xu, Ye 2; Liao, Wenli 2; Zhao, Yajie 1; Zheng, Xiangkuo1; Xu, Chunquan2; Zhou, Tieli2; Cao, Jianming1
发表日期	2020-10-02
发表期刊	ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Escherichia coli Triclosan Efflux pump Resistance fabI Cross-resistance Multidrug-resistance
其他关键词	ANTIBIOTIC-RESISTANCE ; ESCHERICHIA-COLI ; EXPOSURE ; TRICLOCARBAN ; POPULATION
摘要	Background The widespread application of triclosan contributes to its residual deposition in urine, which provides an environment of long-term exposure to triclosan for the intestinalEscherichia coli. We determined the triclosan and antibiotic resistance characteristics ofE. colistrains isolated from urine samples and further investigated the resistance mechanism and molecular epidemic characteristics of triclosan-resistantE. coliisolates. Methods A total of 200 non-repetitiveE. colistrains were isolated from urine samples and then identified. The minimum inhibitory concentrations (MICs) of triclosan and antibiotics,fabImutation, efflux pump activity, the expression of 14 efflux pump encoding genes, and epidemiological characteristics were determined by the agar dilution method, polymerase chain reaction (PCR), carbonyl cyanide 3-chlorophenylhydrazone (CCCP) inhibition test, quantitative real-time polymerase chain reaction (RT-qPCR), multilocus sequence typing (MLST), and pulse-field gel electrophoresis (PFGE) for all triclosan-resistant isolates. Furthermore, we also investigated the effect of triclosan exposure in vitro on antibiotic susceptibility and the efflux pump encoding gene expressions of triclosan-susceptible strains via serial passage experiments. Results Of the 200E. coliisolates, 2.5% (n = 5) were found to be resistant to triclosan, and multidrug resistance (MDR) and cross-resistance phenotypes were noted for these triclosan-resistant strains. The triclosan-sensitive strains also exhibited MDR phenotypes, probably because of the high resistance rate to AMP, CIP, LVX, and GEN. Gly79Ala and Ala69Thr amino acid changes were observed in the triclosan-resistant strains, but these changes may not mediate resistance ofE. colito triclosan, because mutations of these two amino acids has also been detected in triclosan-susceptible strains. Moreover, except for DC8603, all other strains enhanced the efflux pumps activity. As compared with ATCC 25922, except forfabI, increased expressions were noted for all efflux pump encoding genes such asydcV,ydcU,ydcS,ydcT,cysP,yihV,acrB,acrD,andmdfAamong the studied strains with varying PFGE patterns and STs types. Unexpectedly, 5 susceptibleE. coliisolates showed rapidly increasing triclosan resistance after exposure to triclosan in vitro for only 12 days, while MDR or cross-resistance phenotypes and the overexpression of efflux pump genes were recorded among these triclosan-induced resistant isolates. Conclusions This is the first study to report that short-term triclosan exposure in vitro increases triclosan resistance in susceptibleE. coliisolates. After acquiring resistance, these strains may present MDR or cross-resistance phenotypes. Moreover, triclosan resistance mainly involves the overexpression offabIand efflux pumps inE. coliisolates.
资助项目	Planned Science and Technology Project of Wenzhou [Y20180193]
出版者	BMC
出版地	LONDON
ISSN	2047-2994
卷号	9 期号:1 页码:161
DOI	10.1186/s13756-020-00823-5
页数	10
WOS类目	Public, Environmental & Occupational Health ; Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS研究方向	Public, Environmental & Occupational Health ; Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy
WOS记录号	WOS:000576848100001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33008474
PMC记录号	PMC7531082
SCOPUSEID	2-s2.0-85092296288
通讯作者地址	[Zhou, Tieli]Department of Medical Laboratory Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou, Zhejiang Province,China
Scopus学科分类	Public Health, Environmental and Occupational Health;Microbiology (medical);Infectious Diseases;Pharmacology (medical)
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13451
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学
通讯作者	Zhou, Tieli
作者单位	1.Department of Medical Laboratory Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou, Zhejiang Province,China; 2.Department of Clinical Laboratory,Hospital of Wenzhou Medical University,Wenzhou, Zhejiang Province,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Zeng, Weiliang,Xu, Wenya,Xu, Ye,et al. The prevalence and mechanism of triclosan resistance inEscherichia coliisolated from urine samples in Wenzhou, China[J]. ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL,2020,9(1):161.
APA	Zeng, Weiliang., Xu, Wenya., Xu, Ye., Liao, Wenli., Zhao, Yajie., ... & Cao, Jianming. (2020). The prevalence and mechanism of triclosan resistance inEscherichia coliisolated from urine samples in Wenzhou, China. ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 9(1), 161.
MLA	Zeng, Weiliang,et al."The prevalence and mechanism of triclosan resistance inEscherichia coliisolated from urine samples in Wenzhou, China".ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL 9.1(2020):161.