Jiedu Sangen decoction inhibits chemoresistance to 5-fluorouracil of colorectal cancer cells by suppressing glycolysis via PI3K/AKT/HIF-1α signaling pathway

doi:10.1016/S1875-5364(21)60015-8

科研成果详情

题名	Jiedu Sangen decoction inhibits chemoresistance to 5-fluorouracil of colorectal cancer cells by suppressing glycolysis via PI3K/AKT/HIF-1α signaling pathway
作者	SUN, Lei-Tao 1,2; ZHANG, Le-Yin 2; SHAN, Fei-Yu3; SHEN, Min-He 1; RUAN, Shan-Ming 1
发表日期	2021-02
发表期刊	Chinese Journal of Natural Medicines 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Jiedu Sangen decoction 5-Fluorouracil resistance Glycolysis Apoptosis Colorectal cancer
其他关键词	DRUG-RESISTANCE ; HIF-1-ALPHA ; HYPOXIA ; APOPTOSIS ; PI3K/AKT ; CHEMOTHERAPY ; METABOLISM
摘要	Drug resistance is a major obstacle in the development of effective colorectal cancer(CRC) therapy. Our study aimed to explore the reversal abilities of Jiedu Sangen decoction(JSD) on the 5-fluorouracil(5-FU) resistance and its underlying molecular mechanisms. Expression changes in HIF-1 of CRC tissues were firstly revealed by bioinformatics analysis. Afterwards, cell viabilities of JSD and 5-FU treatments on 5-FU resistant human colon cancer cells(HCT-8/5-FU) were determined. Expressions of phosphoinositide 3-kinase(PI3 K), protein kinase B(AKT)/p-AKT, hypoxia-inducible factor 1(HIF-1α), as well as glycolysis related proteins such as L-lactate dehydrogenase A(LDHA), Glucose transporter type 1(Glut1), Hexokinase 2(HKII), and cysteinyl aspartate specific proteinase(Caspase) family members in HCT-8/5-FU cells, HIF-1α silenced HCT-8/5-FU cells and tumor tissues were detected by western blotting. HIF-1α was found over expressed in CRC tissues according to public available datasets in Oncomine. Growth inhibition rates of HCT-8/5-FU cells were increased along with the increase of JSD concentrations. JSD caused down-regulated HIF-1α, PI3 K,AKT/p-AKT, HKII and Glut1, as well as up-regulated Caspase3 and Caspase9 in HCT-8/5-FU cells and tumor tissues. In HIF-1α silenced HCT-8/5-FU cells, synergistic group showed significantly reduced expression levels of PI3 K, AKT, p-AKT. Additionally, upregulated expressions of Caspase6 and Caspase7 were observed. JSD combined with 5-FU also exhibited obvious inhibitory efficiency on tumor growth in vivo. JSD may reverse 5-FU resistance by suppressing glycolysis via PI3 K/AKT/HIF-1α signaling pathway,thereby inhibiting glycolysis and induce apoptosis to enhance anti-tumor activity.
资助项目	supported by General Research Program for Education of Zhejiang Provincial (No. Y202045212);; the Seventh Batch of Provincial Famous Traditional Chinese Medicine Studios in Zhejiang Province;; Cultivation Program for Innovative Talent Graduate Students (No. 721100G00713);; Zhejiang Provincial Program for the Cultivation of the Young and Middle-Aged Academic Leaders in Colleges and Universities (No. 2017-248);; Zhejiang Provincial Project for the Key Discipline of Traditional Chinese Medicine (No. 2017-XK-A09);; Zhejiang Provincial Project for the Science and Technology Program of Traditional Chinese Medicine (No. 2015ZZ013)
出版者	CHINESE JOURNAL NATURAL MEDICINES
出版地	NANJING
ISSN	2095-6975
EISSN	1875-5364
卷号	19 期号:02 页码:143-152
DOI	10.1016/S1875-5364(21)60015-8
页数	10
WOS类目	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000623397400007
收录类别	CNKI ; SCOPUS ; SCIE ; PUBMED ; CSCD
学科领域	医药、卫生 ; 药学
URL	查看原文
CSCD记录号	CSCD:6916508
PubMed ID	33641785
SCOPUSEID	2-s2.0-85101616260
通讯作者地址	[SHEN, Min-He]Department of Medical Oncology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,310006,China
Scopus学科分类	Drug Discovery;Complementary and Alternative Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/134203
专题	附属第一医院_中医科
通讯作者	SHEN, Min-He
作者单位	1.Department of Medical Oncology,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,310006,China; 2.The First Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou,310053,China; 3.Department of Traditional Chinese Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
推荐引用方式 GB/T 7714	SUN, Lei-Tao,ZHANG, Le-Yin,SHAN, Fei-Yu,et al. Jiedu Sangen decoction inhibits chemoresistance to 5-fluorouracil of colorectal cancer cells by suppressing glycolysis via PI3K/AKT/HIF-1α signaling pathway[J]. Chinese Journal of Natural Medicines,2021,19(02):143-152.
APA	SUN, Lei-Tao, ZHANG, Le-Yin, SHAN, Fei-Yu, SHEN, Min-He, & RUAN, Shan-Ming. (2021). Jiedu Sangen decoction inhibits chemoresistance to 5-fluorouracil of colorectal cancer cells by suppressing glycolysis via PI3K/AKT/HIF-1α signaling pathway. Chinese Journal of Natural Medicines, 19(02), 143-152.
MLA	SUN, Lei-Tao,et al."Jiedu Sangen decoction inhibits chemoresistance to 5-fluorouracil of colorectal cancer cells by suppressing glycolysis via PI3K/AKT/HIF-1α signaling pathway".Chinese Journal of Natural Medicines 19.02(2021):143-152.

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