Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis

doi:10.4103/1673-5374.339009

科研成果详情

题名	Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis
作者	Zhang, An-Qi1; Wang, Lu 1; Wang, Yi-Xiu 2; Hong, Shan-Shan1; Zhong, Yu-Shan 1; Yu, Ru-Yi1; Wu, Xin-Lu1; Zhou, Bing-Bing 1; Yu, Qi-Min 1; Fu, Hai-Feng1; Chen, Shuang-Dong 1; Mo, Yun-Chang 1; Dai, Qin-Xue1; Wang, Jun-Lu1
发表日期	2022-03-23
发表期刊	Neural Regeneration Research 影响因子和分区
语种	英语
原始文献类型	Periodical ; Article
关键词	acute ischemic stroke adenosine A1 receptor apoptosis cerebral ischemia-reperfusion injury cortical neurons GATA2 middle cerebral artery occlusion miR-324-3p oxygen-glucose deprivation/reoxygenation PC12 cells
其他关键词	acute ischemic stroke ; adenosine A1 receptor ; apoptosis ; cerebral ischemia-reperfusion injury ; cortical neurons ; GATA2 ; middle cerebral artery occlusion ; miR-324-3p ; oxygen-glucose deprivation/reoxygenation ; PC12 cells ; IN-VITRO ; ADENOSINE ; BRAIN ; STROKE ; BLOOD ; RATS ; EXPRESSION ; APOPTOSIS ; NEURONS ; VIVO
摘要	Previous studies have suggested that miR-324-3p is related to the pathophysiology of cerebral ischemia,but the mechanism underlying this relationship is unclea r.In this study,we found that miR-324-3p expression was decreased in patients with acute ischemic stroke and in in vitro and in vivo models of ischemic stro ke.miR-324-3p agomir potentiated ischemic brain damage in rats subjected to middle cerebral artery occlusion,as indicated by increased infarct volumes and cell apoptosis rates and greater neurological deficits.In a PC12 cell oxygen-glucose deprivation/reoxygenation model,a miR-324-3 p mimic decreased cell viability and expression of the anti-apoptotic protein BCL2 and increased expression of the pro-apoptotic protein BAX and rates of cell apoptosis,whereas treatment with a miR-324-3p inhibitor had the opposite effects.Silencing miR-324-3p increased adenosine A1 receptor(A1R) expression thro ugh regulation of GATA binding protein 2(GATA2).These findings suggest that silencing miR-324-3p reduces ischemic brain damage via the GATA2/A1R axis.
资助项目	funded by the National Natural Science Foundation of China,No.81803937 (to YCM and QXD);; Science and Technology Innovation Activity Plan for College Students of Zhejiang Province (Xinmiao Talent Plan),No.2020R413079 (to AQZ);; Wenzhou Science and Technology Plan Project,No.Y20210122 (to QXD)
出版者	WOLTERS KLUWER MEDKNOW PUBLICATIONS
出版地	MUMBAI
ISSN	1673-5374
EISSN	1876-7958
卷号	17 期号:11 页码:2504-2511
DOI	10.4103/1673-5374.339009
页数	8
WOS类目	Cell Biology ; Neurosciences
WOS研究方向	Cell Biology ; Neurosciences & Neurology
WOS记录号	WOS:000866351500056
收录类别	CNKI ; SCI ; ISTIC ; 万方 ; PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	35535903
SCOPUSEID	2-s2.0-85129885308
通讯作者地址	[Mo, Yun-Chang]Department of Anesthesiology,Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,China ; [Dai, Qin-Xue]Department of Anesthesiology,Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,China ; [Wang, Jun-Lu]Department of Anesthesiology,Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,China
Scopus学科分类	Developmental Neuroscience
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/134180
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_麻醉科
通讯作者	Mo, Yun-Chang; Dai, Qin-Xue; Wang, Jun-Lu
作者单位	1.Department of Anesthesiology,Hospital of Wenzhou Medical University,Zhejiang Province,Wenzhou,China; 2.Department of Anesthesiology,Wenzhou Central Hospital,Zhejiang Province,Wenzhou,China
第一作者单位	麻醉科
通讯作者单位	麻醉科
第一作者的第一单位	麻醉科
推荐引用方式 GB/T 7714	Zhang, An-Qi,Wang, Lu,Wang, Yi-Xiu,et al. Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis[J]. Neural Regeneration Research,2022,17(11):2504-2511.
APA	Zhang, An-Qi., Wang, Lu., Wang, Yi-Xiu., Hong, Shan-Shan., Zhong, Yu-Shan., ... & Wang, Jun-Lu. (2022). Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis. Neural Regeneration Research, 17(11), 2504-2511.
MLA	Zhang, An-Qi,et al."Silencing miRNA-324-3p protects against cerebral ischemic injury via regulation of the GATA2/A1R axis".Neural Regeneration Research 17.11(2022):2504-2511.

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