Proteomic analysis and qRT-PCR verification of Arthrospira platensis strain YZ under dark stress

doi:10.2216/13-167.1

科研成果详情

题名	Proteomic analysis and qRT-PCR verification of Arthrospira platensis strain YZ under dark stress
作者	Zhao, Xiaokai 1; Wang, Xuedong 2; Zhou, Jiaopeng1; Chen, Biaobang 1; Zeng, Aibing 1; Bao, Qiyu1; Wang, Huili1
发表日期	2013-11
发表期刊	PHYCOLOGIA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	2D-PAGE Arthrospira platensis Dark stress MALDI-TOF/MS
其他关键词	CALVIN CYCLE ENZYMES ; LIGHT STRESS ; CIRCADIAN OSCILLATOR ; GENE-EXPRESSION ; RED-LIGHT ; CYANOBACTERIA ; FRUCTOSE-1,6-BISPHOSPHATASE ; PROTEINS ; CHLOROPLAST ; ADAPTATION
摘要	Arthrospira platensis is an ideal organism for a proteome study under conditions of environmental stress. Herein, we report the differential expression of proteins in A. platensis strain YZ responding to 48 h of continuous dark or light conditions by two-dimensional gel electrophoresis and peptide mass fingerprinting techniques. There were 102 proteins retrieved with significant differential expression between light and dark incubation, among which 96 protein spots were found with high homology to Arthrospira genus (67 proteins in A. platensis strain Paraca and 29 in A. maxima strain CS-328). The other six proteins belonged to other microorganisms. Using a clusters of orthologous groups database, we classified the 102 differentially expressed positive proteins into 19 types based on their function and localized them in their respective KEGG metabolic pathways. The proteins were involved in 47 metabolic pathways, such as photosynthesis, glycolysis/gluconeogenesis pathway, oxidative phosphorylation, and amino acid and fatty acid synthesis. These results implied that the proteins performed predictable roles in rendering A. platensis strain YZ resistant against dark stress. Additionally, we determined the transcriptional level of 26 genes in response to dark stress in vivo by quantitative reverse-transcription polymerase chain reaction, and we found that the expression of six differential proteins, representing 23.08% of the total target genes, was consistent between transcriptional and translational levels. The remaining 20 genes showed inconsistent protein expression compared with the transcriptional level, which accounted for 76.92% of the total target genes.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31071115, 31270548]; Natural Science Foundation of Zhejiang Province, ChinaNatural Science Foundation of Zhejiang Province [Y3110050]; Key Project of Zhejiang Environmental Protection Department, China [2012B014]; Initial Foundation of Wenzhou Medical University [QTJ13006]
出版者	ALLEN PRESS INC
出版地	LAWRENCE
ISSN	0031-8884
卷号	52 期号:6 页码:538-549
DOI	10.2216/13-167.1
页数	12
WOS类目	Plant Sciences ; Marine & Freshwater Biology
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000333097500008
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84891137102
通讯作者地址	[Bao, Qiyu]College of Life Sciences,Wenzhou Medical University,China
Scopus学科分类	Aquatic Science;Plant Science
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13388
专题	检验医学院（生命科学学院、生物学实验教学中心）仁济学院_环境与公共卫生学部
通讯作者	Bao, Qiyu
作者单位	1.College of Life Sciences,Wenzhou Medical University,China; 2.School of Environmental Sciences and Public Health,Wenzhou Medical University,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Zhao, Xiaokai,Wang, Xuedong,Zhou, Jiaopeng,et al. Proteomic analysis and qRT-PCR verification of Arthrospira platensis strain YZ under dark stress[J]. PHYCOLOGIA,2013,52(6):538-549.
APA	Zhao, Xiaokai., Wang, Xuedong., Zhou, Jiaopeng., Chen, Biaobang., Zeng, Aibing., ... & Wang, Huili. (2013). Proteomic analysis and qRT-PCR verification of Arthrospira platensis strain YZ under dark stress. PHYCOLOGIA, 52(6), 538-549.
MLA	Zhao, Xiaokai,et al."Proteomic analysis and qRT-PCR verification of Arthrospira platensis strain YZ under dark stress".PHYCOLOGIA 52.6(2013):538-549.