Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach

doi:10.1016/j.cellimm.2018.04.015

科研成果详情

题名	Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach
作者	Zheng, Juzeng1; Ou, Zhanfan 1; Lin, Xianfan1; Wang, Lingling 2; Liu, Yang1; Jin, Sisi1; Wu, Jinming1
发表日期	2018-07
发表期刊	CELLULAR IMMUNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Bioinformatics Epitope Hepatitis B virus E antigen Therapeutic vaccine
其他关键词	T-CELL-EPITOPES ; STRUCTURE PREDICTION ; IMMUNE-RESPONSE ; NEURAL-NETWORKS ; HBV INFECTION ; PEP-FOLD ; PEPTIDES ; HBEAG ; PROTEINS ; CURE
摘要	Chronic hepatitis B virus infection is a worldwide health problem with no current effective strategy to achieve a cure. The Hepatitis B virus (HBV) E antigen (HBeAg) has a negative effect on the immune system and a therapeutic vaccine is a promising strategy in order to treat chronic virus infection. In this study, we analyzed and identified the MHC-I, MHC-II and B cell epitopes of the HBeAg based on a B genotype sequence of HBV using a bioinformatic approach and in vitro experiments. The computational approach provided us with four epitopes (LLWFHISCL, YLVSFGVWI, MQLFHLCLI, TVLEYLVSF) of the specific MHC-I allele HLA-A0201 that conformed to all criteria. Molecular docking and a peptide binding assay showed that epitope TVLEYLVSF had the lowest binding energy and epitope LLWFHISCL had the highest binding affinity to the HLA-A0201 molecule. An interferon.enzyme-linked immunospot assay and cytotoxicity assay revealed that epitope LLWFHISCL had the highest ability to induce and stimulate T cells. Furthermore, we determined four core peptides of MHC-II epitopes and a region of the B cell epitope. The epitopes and region identified in this research may be helpful in designing epitope-based vaccines and boosting the mechanism research of HBeAg and its effect on the immune system.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [Y2110768, LY12H03003]; Technology Bureau of Wenzhou [2014Y0136]
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0008-8749
EISSN	1090-2163
卷号	329 页码:56-65
DOI	10.1016/j.cellimm.2018.04.015
页数	10
WOS类目	Cell Biology ; Immunology
WOS研究方向	Cell Biology ; Immunology
WOS记录号	WOS:000434286200008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29724463
SCOPUSEID	2-s2.0-85046373580
通讯作者地址	[Wu, Jinming]Department of Gastroenterology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13316
专题	附属第一医院其他_附属台州医院（浙江省台州医院）
通讯作者	Wu, Jinming
作者单位	1.Department of Gastroenterology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Gastroenterology,The Affiliated Taizhou Hospital of Wenzhou Medical University,Taizhou,318000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zheng, Juzeng,Ou, Zhanfan,Lin, Xianfan,et al. Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach[J]. CELLULAR IMMUNOLOGY,2018,329:56-65.
APA	Zheng, Juzeng., Ou, Zhanfan., Lin, Xianfan., Wang, Lingling., Liu, Yang., ... & Wu, Jinming. (2018). Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach. CELLULAR IMMUNOLOGY, 329, 56-65.
MLA	Zheng, Juzeng,et al."Analysis of epitope-based vaccine candidates against the E antigen of the hepatitis B virus based on the B genotype sequence: An in silico and in vitro approach".CELLULAR IMMUNOLOGY 329(2018):56-65.