Determination of Diphenhydramine Hydrochloride in Rabbit Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study

科研成果详情

题名	Determination of Diphenhydramine Hydrochloride in Rabbit Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study
作者	Ma, Jianshe1; Zhang, Meiling2; Shentu, Yangping 1; Zhang, Yuqing 1; Fan, Xiaofang1,3; Gong, Yongsheng1,3
发表日期	2011-09
发表期刊	LATIN AMERICAN JOURNAL OF PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Diphenhydramine hydrochloride Liquid chromatography-tandem mass spectrometry Pharmacokinetics Plasma
其他关键词	GAS-LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; PROLONGATION ; ORPHENADRINE ; VALIDATION ; HPLC
摘要	A sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of diphenhydramine hydrochloride in rabbit plasma was developed and validated. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm x 50 mm, 3.5 mu m) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 255.8 -> 0 166.6 for diphenhydramine hydrochloride and m/z 342.9 0 -> 308.0 for the IS. Calibration plots were linear over the range of 5200 ng/mL for diphenhydramine hydrochloride in rabbit plasma. Lower limit of quantitation (LLOQ) for diphenhydramine hydrochloride was 5 ng/mL. RSD of intra-day and inter-day precisions were both less than 10 %. This developed method is successfully used in pharmacokinetic study of diphenhydramine hydrochloride in rabbit.
出版者	COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES
出版地	LA PLATA
ISSN	0326-2383
卷号	30 期号:7 页码:1372-1376
页数	5
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000298220700019
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-82355190837
通讯作者地址	[Gong, Yongsheng]Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China.
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13091
专题	基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Gong, Yongsheng
作者单位	1.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China; 2.Wenzhou Med Coll, Analyt & Testing Ctr, Wenzhou 325035, Peoples R China; 3.Wenzhou Med Coll, Inst Hypoxia Med, Wenzhou 325035, Peoples R China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Ma, Jianshe,Zhang, Meiling,Shentu, Yangping,et al. Determination of Diphenhydramine Hydrochloride in Rabbit Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2011,30(7):1372-1376.
APA	Ma, Jianshe, Zhang, Meiling, Shentu, Yangping, Zhang, Yuqing, Fan, Xiaofang, & Gong, Yongsheng. (2011). Determination of Diphenhydramine Hydrochloride in Rabbit Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study. LATIN AMERICAN JOURNAL OF PHARMACY, 30(7), 1372-1376.
MLA	Ma, Jianshe,et al."Determination of Diphenhydramine Hydrochloride in Rabbit Plasma by LC-MS/MS and its Application to a Pharmacokinetic Study".LATIN AMERICAN JOURNAL OF PHARMACY 30.7(2011):1372-1376.