Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension

doi:10.1186/s12920-020-00740-x

科研成果详情

题名	Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension
作者	Luo, Jing 1; Li, Haiyan2,3; Liu, Zhenwei4; Li, Chenlu 1; Wang, Ruochen4; Fang, Jinxia 1; Lu, Saisai 1; Guo, Jing 5; Zhu, Xiaochun1; Wang, Xiaobing1
发表日期	2020-06-26
发表期刊	BMC MEDICAL GENOMICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Pulmonary arterial hypertension Differentially expressed gene Functional enrichment analysis Protein-protein interaction network miRNAs
其他关键词	MONOCROTALINE PYRROLE ; CENP-E ; KINASE ; CDK1 ; PROTEIN ; IDENTIFICATION ; PROLIFERATION ; ACTIVATION ; MUTATIONS ; CYTOSCAPE
摘要	Background Pulmonary arterial hypertension (PAH) is a life-threatening condition. The aim of this study was to explore potential crucial genes and pathways associated with PAH based on integrative analyses of gene expression and to shed light on the identification of biomarker for PAH. Methods Gene expression profile of pulmonary tissues from 27 PAH patients and 22 normal controls were downloaded from public database (GSE53408 and GSE113439). After the identification of differentially expressed genes (DEGs), hub pathways and genes were identified based on the comprehensive evaluation of protein-protein interaction (PPI) network analysis, modular analysis and cytohubba's analysis, and further validated in another PAH transcriptomic dataset (GSE33463). Potentially associated micro-RNAs (miRNAs) were also predicted. Results A total of 521 DEGs were found between PAH and normal controls, including 432 up-regulated DEGs and 89 down-regulated DEGs. Functional enrichment analysis showed that these DEGs were mainly enriched in mitotic cell cycle process, mitotic cell cycle and microtubule cytoskeleton organization. Moreover, five key genes (CDK1, SMC2, SMC4, KIF23,andCENPE) were identified and then further validated in another transcriptomic dataset associated with special phenotypes of PAH. Furthermore, these hub genes were mainly enriched in promoting mitotic cell cycle process, which may be closely associated with the pathogenesis of PAH. We also found that the predicted miRNAs targeting these hub genes were found to be enriched in TGF-beta and Hippo signaling pathway. Conclusion These findings are expected to gain a further insight into the development of PAH and provide a promising index for the detection of PAH.
资助项目	National Science FoundationNational Science Foundation (NSF) [81700062]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LQ16H010003]; Science and Technology Project of Zhejiang Provincial Health Commission [2019RC050]; Zhejiang Xinmiao Talents Program [2019R413082]; General scientific projects of Zhejiang Education Department [Y201942208]
出版者	BMC
出版地	LONDON
ISSN	1755-8794
EISSN	1755-8794
卷号	13 期号:1 页码:86
DOI	10.1186/s12920-020-00740-x
页数	14
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000545758100002
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32586319
PMC记录号	PMC7318763
SCOPUSEID	2-s2.0-85087140744
通讯作者地址	[Wang, Xiaobing]Wenzhou Med Univ, Affiliated Hosp 1, Rheumatol Dept, Wenzhou 325035, Peoples R China.
Scopus学科分类	Genetics;Genetics (clinical)
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12962
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院基因组医学研究院精神医学学院_心理学系
通讯作者	Wang, Xiaobing
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Rheumatol Dept, Wenzhou 325035, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 2, Dept Pediat Pulmonol, Wenzhou, Peoples R China; 3.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Peoples R China; 4.Wenzhou Med Univ, Inst Genom Med, Wenzhou, Peoples R China; 5.Wenzhou Med Univ, Coll Psychol Med, Wenzhou, Peoples R China
第一作者单位	附属第一医院; 风湿免疫科
通讯作者单位	附属第一医院; 风湿免疫科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Luo, Jing,Li, Haiyan,Liu, Zhenwei,et al. Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension[J]. BMC MEDICAL GENOMICS,2020,13(1):86.
APA	Luo, Jing., Li, Haiyan., Liu, Zhenwei., Li, Chenlu., Wang, Ruochen., ... & Wang, Xiaobing. (2020). Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension. BMC MEDICAL GENOMICS, 13(1), 86.
MLA	Luo, Jing,et al."Integrative analyses of gene expression profile reveal potential crucial roles of mitotic cell cycle and microtubule cytoskeleton in pulmonary artery hypertension".BMC MEDICAL GENOMICS 13.1(2020):86.