Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients

doi:10.1007/s13577-021-00507-1

科研成果详情

题名	Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients
作者	Lin, Lizhi1; Wen, Jialiang 1; Lin, Bangyi 1; Xia, Erjie 1; Zheng, Chen1; Ye, Lechi2; Wang, Yinghao 1; Wang, Ouchen1; Chen, Yizuo1
发表日期	2021-05
发表期刊	HUMAN CELL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Stress-induced phosphoprotein 1 (STIP1) Breast cancer Progression Biomarker JAK2 STAT3 pathway
其他关键词	PROMOTES TUMOR-GROWTH ; HEPATOCELLULAR-CARCINOMA ; METASTASIS ; EXPRESSION ; MIGRATION ; STIP1 ; PROLIFERATION ; CHAPERONES ; BIOMARKER ; HSP70
摘要	Breast cancer (BC) threatened the life health of a tremendous amount of the population, and the estimated number of death is still rising nowadays. We found that stress-induced phosphoprotein 1 (STIP1) is overexpressed in BC tissues compared to non-tumorous breast tissues. Our study is to validate the prognostic value of STIP1 and investigate its biological role in BC. We verified the upregulation of STIP1 in multiple databases, proved that STIP1 is upregulated in BC tissues and cell lines using real-time quantitative PCR (qRT-PCR). We used small interfering RNA to examine the function of STIP1 in BC cell lines (BT-549, MDA-MB-231, Hs-578 T) and explored the mechanism of function of STIP1 in BC cells using Western blotting and qRT-PCR. Analyses of multiple databases indicated that high STIP1 expression is a marker that effectively distinguishes BC patients from healthy control and predicts worse clinical outcomes in BC. The loss-of-function experiments showed that STIP1 silencing results in inhibition of cell proliferation and migration, inducing cell apoptosis, and S-phase arrest in vitro. Our study also showed that STIP1 downregulation inhibited the JAK2/STAT3 pathway and epithelial-mesenchymal transition process. Rescue experiments demonstrated that the oncogenic effect of STIP1 is partially dependent on mediating JAK2 expression. This study verified that STIP1 is an oncogenic gene that promotes BC progression and serves as a valuable diagnostic and outcome-related marker of BC.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81572291]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LGF18H160031, LY18H160053]; Science And Technology Project of Wenzhou [Y20190204, Y20170740]
出版者	SPRINGER JAPAN KK
出版地	TOKYO
ISSN	0914-7470
EISSN	1749-0774
卷号	34 期号:3 页码:901-917
DOI	10.1007/s13577-021-00507-1
页数	17
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000625809500001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	33665786
SCOPUSEID	2-s2.0-85102200218
通讯作者地址	[Chen, Yizuo]Department of Thyroid and Breast Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Cell Biology;Cancer Research
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12944
专题	附属第一医院_肛肠外科
通讯作者	Chen, Yizuo
作者单位	1.Department of Thyroid and Breast Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Colorectal and Anal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Lizhi,Wen, Jialiang,Lin, Bangyi,et al. Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients[J]. HUMAN CELL,2021,34(3):901-917.
APA	Lin, Lizhi., Wen, Jialiang., Lin, Bangyi., Xia, Erjie., Zheng, Chen., ... & Chen, Yizuo. (2021). Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients. HUMAN CELL, 34(3), 901-917.
MLA	Lin, Lizhi,et al."Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients".HUMAN CELL 34.3(2021):901-917.