题名 | The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382 |
作者 | |
发表日期 | 2017-12-31 |
发表期刊 | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Lncrna-TUG1 MiR-382 EZH2 Pancreatic cancer Proliferation Migration EMT |
其他关键词 | DUCTAL ADENOCARCINOMA GROWTH ; MESENCHYMAL TRANSITION ; OVEREXPRESSION ; EXPRESSION |
摘要 | Background/Aims: Pancreatic carcinoma (PC) is the one of the most common and malignant cancers worldwide. LncRNA taurine upregulated gene 1 (TUG1) was initially identified as a transcript upregulated by taurine, and the abnormal expression of TUG1 has been reported in many cancers. However, the biological role and molecular mechanism of TUG1 in PC still needs further investigation. Methods: Quantitative real-time PCR (qRT-PCR) was performed to measure the expression of TUG1 in PC cell lines and tissues. MTT and colony formation assays were used to measure the effect of TUG1 on cell proliferation. A wound healing assay, transwell assay and western blot assay were employed to determine the effect of TUG1 on cell migration and the epithelial mesenchymal transition (EMT) phenotype. RNA-binding protein immunoprecipitation (RIP) and a biotin-avidin pulldown system were performed to confirm the interaction between miR-328 and TUG1. A gene expression array analysis using clinical samples and RT-qPCR suggested that enhancer of zeste homolog 2 (EZH2) was a target of miR-382 in PC. Results: In this study, we reported that TUG1 was overexpressed in PC tissues and cell lines, and high expression of TUG1 predicted poor prognosis. Further experiments revealed that overexpressed TUG1 promoted cell proliferation, migration and contributed to EMT formation, whereas silenced TUG1 led to opposing results. Additionally, luciferase reporter assays, an RIP assay and an RNA-pulldown assay demonstrated that TUG1 could competitively sponge miR-382 and thereby regulate EZH2. Conclusion: Collectively, these findings revealed that TUG1 functions as an oncogenic IncRNA that promotes tumor progression, at least partially, by functioning as an endogenous 'sponge' and competing for miR-382 binding to the miRNA target EZH2. (C) 2017 The Author(s) Published by S. Karger AG, Basel |
资助项目 | technology innovation team of diagnosis and treatment of abdominal surgery of Wenzhou, Zhejiang Province, China [2016-354]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY15H160056]; [WKJ-ZJ-1706] |
出版者 | KARGER |
出版地 | BASEL |
ISSN | 1015-8987 |
EISSN | 1421-9778 |
卷号 | 42期号:6页码:2145-2158 |
DOI | 10.1159/000479990 |
页数 | 14 |
WOS类目 | Cell Biology ; Physiology |
WOS研究方向 | Cell Biology ; Physiology |
WOS记录号 | WOS:000415239800001 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 28813705 |
SCOPUSEID | 2-s2.0-85027698463 |
通讯作者地址 | [Zhou, Mengtao]Wenzhou Med Univ, Affiliated Hosp 1, Dept Surg, 2 FuXue Lane, Wenzhou, Zhejiang, Peoples R China. |
Scopus学科分类 | Physiology |
TOP期刊 | TOP期刊 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/12865 |
专题 | 第一临床医学院(信息与工程学院)、附属第一医院_外科学_外科实验室 附属第一医院 |
通讯作者 | Zhou, Mengtao |
作者单位 | 1.Wenzhou Med Univ, Affiliated Hosp 1, Key Lab Surg, 2 FuXue Lane, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp 1, Dept Surg, 2 FuXue Lane, Wenzhou, Zhejiang, Peoples R China |
第一作者单位 | 第一临床医学院(信息与工程学院)、附属第一医院_外科学_外科实验室 |
通讯作者单位 | 附属第一医院 |
第一作者的第一单位 | 第一临床医学院(信息与工程学院)、附属第一医院_外科学_外科实验室 |
推荐引用方式 GB/T 7714 | Zhao, Liang,Sun, Hongwei,Kong, Hongru,et al. The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2017,42(6):2145-2158. |
APA | Zhao, Liang, Sun, Hongwei, Kong, Hongru, Chen, Zongjing, Chen, Bicheng, & Zhou, Mengtao. (2017). The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 42(6), 2145-2158. |
MLA | Zhao, Liang,et al."The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 42.6(2017):2145-2158. |
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