Effects of COX-2 inhibitor on ventilator-induced lung injury in rats

doi:10.1016/j.intimp.2013.03.031

科研成果详情

题名	Effects of COX-2 inhibitor on ventilator-induced lung injury in rats
作者	Jin, Li-da1; Wang, Liang-rong1; Wu, Li-qin2; Shan, Yuan-lu1; Zhao, Xi-yue1; Xiong, Xiang-qing1; Zhou, Jun-hui 1; Lin, Li-na1; Jin, Lie-lie1
发表日期	2013-06
发表期刊	INTERNATIONAL IMMUNOPHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Mechanical ventilation Lung injury Aquaporin Cyclooxygenase inhibitors
其他关键词	NECROSIS-FACTOR-ALPHA ; NF-KAPPA-B ; ISOLATED MOUSE LUNGS ; MECHANICAL VENTILATION ; EPITHELIAL-CELLS ; DOWN-REGULATION ; MESSENGER-RNA ; AQUAPORIN-3 EXPRESSION ; ACUTE-PANCREATITIS ; PULMONARY-EDEMA
摘要	Background: Mechanical ventilation especially with large tidal volume has been demonstrated to activate inflammatory response inducing lung injury, which could be attenuated by cyclooxygenase (COX)-2 inhibitors. As the main small integral membrane proteins that selectively conduct water molecules' transportation, aquaporin (AQP)-1 downregulation significantly related to lung edema and inflammation. This study aims to investigate the role of AQP1 in ventilator-induced lung injury in rats and evaluates the effects of COX-2 inhibition. Methods: Forty rats were allocated into four groups, where rats in Groups LD (low volume + DMSO) and LN (low volume + NS-398) were given intravenously 2 ml DMSO and 8 mg/kg NS-398 (a specific COX-2 inhibitor, dissolved in 2 ml DMSO) before 4-hour lower tidal volume ventilation (8 ml/kg), respectively, while DMSO and NS-398 were administrated in the same manner before 4-hour injurious ventilation (40 ml/kg) in Groups HD (high volume + DMSO) and HN (high volume + NS-398). The arachidonic acid metabolites (6-keto prostaglandin F1 alpha, thromboxane B-2), inflammatory cytokines (tumor necrosis factor-at, interleukin-1 beta, 6,8) and total protein levels in bronchoalveolar lavage (BAL) fluid and COX-2 mRNA and AQP1 protein expression in lung tissue were detected; water content and lung morphology were also evaluated. Results: Compared to Groups LD and LN, the rats in Groups HD and HN suffered obvious lung morphological changes with higher wet-to-dry weight ratio and lung injury score, and the levels of arachidonic acid metabolites, inflammatory cytokines and total protein in BAL fluid were increased, the expression of COX-2 mRNA was significantly upregulated and AQP1 protein was downregulated in lung tissue (p < 0.05). The changes in BAL fluid and the severity of lung injury were attenuated, and AQP1 expression was upregulated in Group HN as compared to HD (p < 0.05). Conclusions: Ventilation with large tidal volume causes inflammatory mediator production and AQP1 downregulation, which could be attenuated by COX-2 inhibition. (c) 2013 Elsevier B.V. All rights reserved.
资助项目	Science and Technology Bureau of Wenzhou City, Zhejiang Province, China [Y20100025]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	1567-5769
EISSN	1878-1705
卷号	16 期号:2 页码:288-295
DOI	10.1016/j.intimp.2013.03.031
页数	8
WOS类目	Immunology ; Pharmacology & Pharmacy
WOS研究方向	Immunology ; Pharmacology & Pharmacy
WOS记录号	WOS:000320895400024
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	23587488
通讯作者地址	[Lin, Li-na]Wenzhou Med Coll, Affiliated Hosp 1, Dept Anesthesiol, Wenzhou 325000, Zhejiang, Peoples R China.
TOP期刊	TOP期刊
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12736
专题	附属第一医院附属第二医院附属第二医院_呼吸内科附属第一医院_麻醉科第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Lin, Li-na
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Anesthesiol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Affiliated Hosp 2, Dept Resp Med, Wenzhou, Peoples R China
第一作者单位	附属第一医院; 麻醉科
通讯作者单位	附属第一医院; 麻醉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Jin, Li-da,Wang, Liang-rong,Wu, Li-qin,et al. Effects of COX-2 inhibitor on ventilator-induced lung injury in rats[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2013,16(2):288-295.
APA	Jin, Li-da., Wang, Liang-rong., Wu, Li-qin., Shan, Yuan-lu., Zhao, Xi-yue., ... & Jin, Lie-lie. (2013). Effects of COX-2 inhibitor on ventilator-induced lung injury in rats. INTERNATIONAL IMMUNOPHARMACOLOGY, 16(2), 288-295.
MLA	Jin, Li-da,et al."Effects of COX-2 inhibitor on ventilator-induced lung injury in rats".INTERNATIONAL IMMUNOPHARMACOLOGY 16.2(2013):288-295.