bFGF enhances activation of osteoblast differentiation and osteogenesis on titanium surfaces via PI3K/Akt signaling pathway

科研成果详情

题名	bFGF enhances activation of osteoblast differentiation and osteogenesis on titanium surfaces via PI3K/Akt signaling pathway
作者	Zheng, An-Qi1,2; Xiao, Jian3; Xie, Jing1; Lu, Pei-Pei 1; Ding, Xi1
发表日期	2016-12-31
发表期刊	INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Titanium (Ti) osteoblast basic fibroblast growth factor (bFGF) PI3K/Akt signaling pathway
其他关键词	BONE MORPHOGENIC PROTEIN-2 ; FIBROBLAST GROWTH-FACTORS ; FGF ; INHIBITION ; RECEPTORS ; IMPLANT ; ROLES ; CELLS ; PROLIFERATION ; MANAGEMENT
摘要	Objectives: The aim of this study was to investigate the role of bFGF during the differentiation of osteoblasts and in the course of osteogenesis on titanium surfaces. Materials and methods: Basic f.ibroblast growth factor (bFGF, FGF-2), a member of the fibroblast growth factor family, has many biological effects on cell growth, differentiation, and survival. It was added into this study and MC3T3-E1 Subclone14 was cultivated. The MTT method was used to detect the proliferation of osteoblasts. Alkaline phosphatase (ALP) and osteocalcin (OCN) were examined for indicators of osteoblast differentiation and osteogenesis. Immunofluorescence staining was used to observe protein fluorescence intensity and the Western-blot method was used to analyze the change of the proteins and study the PI3K/Akt signaling pathway. Results: The experimental results showed that the OD values of the MTT, ALP, and OCN rose and the expression levels of OPG, Runx2, and BMP-2 proteins increased when bFGF was added. Moreover, the concentration of 40 ng/ml bFGF was the optimum concentration. Furthermore, the results of signaling pathway analysis indicated that bFGF could promote expression of OPG, Runx2, p-Akt, and BMP-2 proteins via an activated PI3K/Akt signaling pathway. Conclusion: A certain concentration of bFGF could promote the proliferation, differentiation, and osteogenesis of osteoblasts on Ti surfaces via an activated PI3K/Akt signaling pathway.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81000461]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2100274]; Wenzhou Science and Technology Bureau projects [Y20140699]
出版者	E-CENTURY PUBLISHING CORP
出版地	MADISON
ISSN	1936-2625
卷号	9 期号:4 页码:4680-4692
页数	13
WOS类目	Oncology ; Pathology
WOS研究方向	Oncology ; Pathology
WOS记录号	WOS:000377601900047
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84969506763
通讯作者地址	[Ding, Xi]Department of Stomatology,The First Affiliated Hospital,Wenzhou Medical University,Nanbaixiang, Ouhai District, Wenzhou, Zhejiang,325000,China
Scopus学科分类	Pathology and Forensic Medicine;Histology
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12710
专题	药学院（分析测试中心）_生物制药系_生物制药工程附属第一医院研究生工作部（研究生院）口腔医学院、附属口腔医院_儿童口腔
通讯作者	Ding, Xi
作者单位	1.Department of Stomatology,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang,China; 2.Department of Pediatric Dentistry,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou, Zhejiang,China; 3.School of Pharmacy,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou, Zhejiang,China
第一作者单位	附属第一医院; 口腔医学院、附属口腔医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zheng, An-Qi,Xiao, Jian,Xie, Jing,et al. bFGF enhances activation of osteoblast differentiation and osteogenesis on titanium surfaces via PI3K/Akt signaling pathway[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2016,9(4):4680-4692.
APA	Zheng, An-Qi, Xiao, Jian, Xie, Jing, Lu, Pei-Pei, & Ding, Xi. (2016). bFGF enhances activation of osteoblast differentiation and osteogenesis on titanium surfaces via PI3K/Akt signaling pathway. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 9(4), 4680-4692.
MLA	Zheng, An-Qi,et al."bFGF enhances activation of osteoblast differentiation and osteogenesis on titanium surfaces via PI3K/Akt signaling pathway".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 9.4(2016):4680-4692.