Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model

doi:10.1371/journal.pone.0160942

科研成果详情

题名	Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model
作者	Zheng, Jun; Xi, Shanshan; Ding, Maochao; Li, Hong; Xu, Wei; Tang, Maolin; Chen, Shixin
发表日期	2016-08-11
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	ISLAND SKIN FLAP ; BLOOD-FLOW ; INFLOW ; VESSEL ; AUGMENTATION ; EXPRESSION ; DRAINAGE ; SYSTEM
摘要	Objective To comparatively assess the effects of venous superdrainage and arterial supercharging on dorsal perforator flap survival. Materials and Methods Sixty male Sprague-Dawley rats (450-550g) were randomly divided into three groups (n = 20), including control group (Control) and experimental groups A (venous superdrainage, Exp. A) and B (arterial supercharging, Exp. B). At postoperative day 7, survival areas of the flaps were evaluated and all animals underwent angiography. Laser Doppler was used to evaluate flap perfusion from 0h to 7days after surgery. Histology with hematoxylin and eosin staining was used to count microvessels. Tissue of Choke vessels was excised for quantification of hypoxia inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) by western blot assay at 6h and 7days after surgery. Results In the Exp. A group, almost all flaps survived (98.2 +/- 1.6%); in the Exp. B and control group, survival areas accounted for 78.8 +/- 8.5% and 60.3 +/- 7.8%, respectively (P < 0.001). In addition, Exp. A animals showed improved anastomosis of choke vessels 2 compared with the Exp. B and Control groups. Furthermore, flap blood flow and partial pressure of oxygen in the Exp. A group were significantly higher compared with values obtained for the Exp. B and Control groups, from 6 hours to 7 days after surgery. More microvessels were found in the Exp. A group (11.65 +/- 1.33) than in Exp. B (9.25 +/- 0.34) and control (7.25 +/- 0.91) animals on POD 7. The relative expression level of HIF-1 alpha and VEGF were significant at 6h and 7days after surgery. Conclusions Venous superdrainage in rat dorsal perforator flap is more effective than arterial supercharging in promoting flap survival, and could effectively alter hemodynamics in the microcirculation and stimulate blood vessel formation.
资助项目	National Natural Science Fundation of ChinaNational Natural Science Foundation of China (NSFC) [31371214]; Natural Fundation of Zhejiang Province [Y12C110007]; College Sthdents' Activity Plan of ZhejiangProvince [2014R413030]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	11 期号:8 页码:e0160942.
DOI	10.1371/journal.pone.0160942
页数	10
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000381381100091
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	27513520
PMC记录号	PMC4981354
SCOPUSEID	2-s2.0-84983504214
通讯作者地址	[Chen, Shixin]Wenzhou Med Univ, Dept Human Anat, Wenzhou, Peoples R China.
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Agricultural and Biological Sciences (all);Multidisciplinary
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12509
专题	基础医学院（机能实验教学中心）_形态学系_人体解剖学
通讯作者	Chen, Shixin
作者单位	Wenzhou Med Univ, Dept Human Anat, Wenzhou, Peoples R China
第一作者单位	基础医学院（机能实验教学中心）_形态学系_人体解剖学
通讯作者单位	基础医学院（机能实验教学中心）_形态学系_人体解剖学
第一作者的第一单位	基础医学院（机能实验教学中心）_形态学系_人体解剖学
推荐引用方式 GB/T 7714	Zheng, Jun,Xi, Shanshan,Ding, Maochao,et al. Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model[J]. PLOS ONE,2016,11(8):e0160942..
APA	Zheng, Jun., Xi, Shanshan., Ding, Maochao., Li, Hong., Xu, Wei., ... & Chen, Shixin. (2016). Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model. PLOS ONE, 11(8), e0160942..
MLA	Zheng, Jun,et al."Effects of Venous Superdrainage and Arterial Supercharging on Dorsal Perforator Flap in a Rat Model".PLOS ONE 11.8(2016):e0160942..