Toxicological Assessment of Trace beta-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis

doi:10.1371/journal.pone.0102731

科研成果详情

题名	Toxicological Assessment of Trace beta-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis
作者	Yin, Xiaohan 1; Wang, Huili2; Zhang, Yuna 1; Dahlgren, Randy A.1; Zhang, Hongqin2; Shi, Mengru2; Gao, Ming1; Wang, Xuedong 1
发表日期	2014-07-25
发表期刊	PLOS ONE 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	MIDBRAIN-HINDBRAIN BOUNDARY ; GTP-BINDING PROTEINS ; WASTE-WATER ; IDENTIFICATION ; TETRACYCLINES ; GENOTOXICITY ; EXPRESSION ; USAGE ; DRG
摘要	beta-Diketone antibiotics (DKAs) can produce chronic toxicity in aquatic ecosystems due to their pseudo-persistent in the environment. In this study, after long-term DKA exposure to zebrafish (Danio rerio), 47 protein spots had greater than 2-fold differential expression as compared to the control; there were 26 positive proteins with 14 up-regulated and 12 down-regulated. The main functions of the differentially expressed proteins were related to signal transduction mechanisms and the cytoskeleton. Of the 26 target genes, 11 genes were consistent between their transcriptional and translational levels. Low dose DKA exposure (4.69 and 9.38 mg/L) stimulated spontaneous movement in zebrafish. Changes in both creatine kinase activity and creatine concentration showed a similar trend to zebrafish activity. There was no obvious change in SV-BA after DKA exposure, while a reduction of heart rate was concomitant with increasing DKA concentrations. DKAs also induced severe histopathological changes in zebrafish heart tissue, such as dissolution of cristae and vacuolation of mitochondria. These results demonstrated that trace-level DKA exposure affects a variety of cellular and biological processes in zebrafish.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270548, 21377100]; Key Project of Zhejiang Provincial Environmetal Protetion Department [2012B014]; Science and Technology Project of Wenzhou City [20100299]
出版者	PUBLIC LIBRARY SCIENCE
出版地	SAN FRANCISCO
ISSN	1932-6203
卷号	9 期号:7 页码:e102731.
DOI	10.1371/journal.pone.0102731
页数	12
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000339992600020
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25062015
PMC记录号	PMC4111491
SCOPUSEID	2-s2.0-84904866502
通讯作者地址	[Wang, Huili]Wenzhou Med Univ, Coll Life Sci, Key Lab Wenzhou Marine Biol, Wenzhou, Peoples R China.
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Agricultural and Biological Sciences (all);Multidisciplinary
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12335
专题	公共卫生学院_水环境应用技术研究所检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Wang, Huili
作者单位	1.Wenzhou Med Univ, Inst Wenzhou Appl Technol Environm Res, Wenzhou, Peoples R China; 2.Wenzhou Med Univ, Coll Life Sci, Key Lab Wenzhou Marine Biol, Wenzhou, Peoples R China
第一作者单位	水环境应用技术研究所
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	水环境应用技术研究所
推荐引用方式 GB/T 7714	Yin, Xiaohan,Wang, Huili,Zhang, Yuna,et al. Toxicological Assessment of Trace beta-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis[J]. PLOS ONE,2014,9(7):e102731..
APA	Yin, Xiaohan., Wang, Huili., Zhang, Yuna., Dahlgren, Randy A.., Zhang, Hongqin., ... & Wang, Xuedong. (2014). Toxicological Assessment of Trace beta-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis. PLOS ONE, 9(7), e102731..
MLA	Yin, Xiaohan,et al."Toxicological Assessment of Trace beta-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis".PLOS ONE 9.7(2014):e102731..